ABSTRACT This study was to investigate the effects of peptides derived from fermented soybean meal (FSP) on intestinal inflammation and epithelial barrier function of the piglets. Compared to fed soybean meal, piglets fed with fermented soybean meal (FSBM) exhibit lower serum levels of IL-6, IL-1β and D-lactate, and reduced ileum macrophage infiltration. Besides, the FSBM up-regulated (p < 0.05) expression of mucins and tight junction (TJ) proteins in the jejunum and ileum of piglets. The FSP were prepared with alkali extraction and a 3-kDa molecular weight cut-off membrane system. In vitro, FSP reducing the mRNA expression of IL-6, IL-1β, IL-8 and attenuated Escherichia coli K88-induced inflammation in the intestinal porcine epithelial cells-J2 (IPEC-J2). Furthermore, FSP increased (p < 0.05) the expression of TJ proteins and transepithelial electrical resistance in IPEC-J2 cells. These results suggest that FSP could improve small intestinal health by enhancing epithelial barrier function and suppressing inflammation.

中文翻译：

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发酵豆粕肽抑制仔猪肠道炎症并增强上皮屏障功能

摘要 本研究旨在研究发酵豆粕 (FSP) 衍生肽对仔猪肠道炎症和上皮屏障功能的影响。与饲喂豆粕相比，饲喂发酵豆粕 (FSBM) 的仔猪血清 IL-6、IL-1β 和 D-乳酸水平较低，回肠巨噬细胞浸润减少。此外，FSBM 上调 (p < 0.05) 仔猪空肠和回肠中粘蛋白和紧密连接 (TJ) 蛋白的表达。FSP 是用碱提取和 3-kDa 分子量截留膜系统制备的。在体外，FSP 降低 IL-6、IL-1β、IL-8 的 mRNA 表达并减轻大肠杆菌 K88 诱导的猪肠上皮细胞-J2 (IPEC-J2) 的炎症。此外，FSP 增加（p < 0。05) IPEC-J2细胞中TJ蛋白的表达和跨上皮电阻。这些结果表明 FSP 可以通过增强上皮屏障功能和抑制炎症来改善小肠健康。