ABSTRACT The work focused on the study of the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. The diet of experimental chicks was enriched with 0.8% of HS. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens.

中文翻译：

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腐殖质对肉鸡肠道菌群及免疫反应的影响

摘要 这项工作的重点是研究腐殖质 (HS) 对肉鸡的免疫调节和肠道保护作用。实验雏鸡的饮食富含 0.8% 的 HS。我们注意到HS对吞噬细胞的吞噬活性和吞噬能力有刺激作用，但是，吞噬细胞的氧化爆发水平不受影响。我们观察到 CD4+：CD8+ 淋巴细胞比率显着增加，这是免疫刺激的指标。HS不影响IgA基因表达。相比之下，我们观察到 MUC-2（肠道粘蛋白 2）基因表达显着增加，IGF-2（胰岛素样生长因子 2）和 AvBD2（禽β防御素 2）基因表达减少. 实验动物肠道中肠杆菌科细菌数量的减少显示出对肠道微生物群的积极影响。我们证实了 HS 在肉鸡中的肠道保护和免疫刺激作用。