ABSTRACT A monoclonal antibody against carazolol (CRZ), 2F5, was generated for the construction of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip for detection of CRZ in milk and pig kidney samples. The antibody had a half-maximal inhibitory concentration of 0.29 ng/mL; its limit of detection was 0.068 ng/mL, and its linear range of detection was 0.137–0.638 ng/mL. For CRZ in milk samples and porcine kidney samples, the detection limits for this strip were 0.25 and 2.5 ng/mL, respectively. Analysis of CRZ in milk samples showed that the results obtained by immunochromatographic strip were consistent with those obtained by ic-ELISA. Therefore, the CG immunoassay is a sensitive screening method for semi-quantitative and qualitative detection of residual CRZ in milk and porcine kidney samples.

中文翻译：

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用于检测 β-受体阻滞剂卡拉唑醇的 ic-ELISA 和胶体金试纸的开发

摘要 产生抗卡拉唑醇 (CRZ) 的单克隆抗体 2F5，用于构建间接竞争性酶联免疫吸附试验 (ic-ELISA) 和用于检测牛奶和猪肾样品中 CRZ 的免疫层析条。该抗体的半数抑制浓度为 0.29 ng/mL；其检出限为0.068 ng/mL，线性检测范围为0.137-0.638 ng/mL。对于牛奶样品和猪肾样品中的 CRZ，该试纸的检测限分别为 0.25 和 2.5 ng/mL。牛奶样品中CRZ的分析表明，免疫层析法检测结果与ic-ELISA检测结果一致。因此，CG 免疫测定是一种灵敏的筛选方法，可用于半定量和定性检测牛奶和猪肾样品中残留的 CRZ。