ABSTRACT In this work, broad-specific monoclonal antibody based immunoaffinity columns (IACs) coupled to ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for purification and determination of T-2 and HT-2 toxin in maize and cherry samples. Based on broad-specific anti-T-2 monoclonal antibody, the prepared IACs could be used for both T-2 and HT-2 purification with cross reactivity values at 100% for T-2 and 108% for HT-2. Column capacity was 3.14 μg mL−1 gel for T-2 toxin, and 3.20 μg mL−1 gel for HT-2 toxin. After 10 cycles of usage at intervals of 2 days, column capacity decreased to 0.85 μg mL−1 gel for T-2 and 1.22 μg mL−1 gel for HT-2 toxin. The developed method exhibited high sensitive results with limit of detection (LOD) lower than 0.35 μg kg−1 and 1.42 μg kg−1.

中文翻译：

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基于广谱特异性单克隆抗体的 IAC 纯化耦合 UPLC-MS/MS 方法，用于测定玉米和樱桃样品中的 T-2 和 HT-2 毒素

摘要 在这项工作中，基于广谱特异性单克隆抗体的免疫亲和柱 (IAC) 与超高效液相色谱串联质谱 (UPLC-MS/MS) 联用，用于纯化和测定玉米和玉米中的 T-2 和 HT-2 毒素。樱桃样品。基于广谱特异性抗 T-2 单克隆抗体，制备的 IAC 可用于 T-2 和 HT-2 纯化，T-2 的交叉反应性值为 100%，HT-2 的交叉反应性值为 108%。T-2 毒素的柱容量为 3.14 μg mL-1 凝胶，HT-2 毒素的柱容量为 3.20 μg mL-1 凝胶。以 2 天为间隔使用 10 次循环后，T-2 的柱容量降至 0.85 μg mL-1 凝胶，HT-2 毒素的柱容量降至 1.22 μg mL-1 凝胶。开发的方法表现出高灵敏度的结果，检测限 (LOD) 低于 0.35 μg kg-1 和 1.42 μg kg-1。