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Label-free rapid identification of cooked meat using MIP-quantum weak measurement
Food and Agricultural Immunology ( IF 1.7 ) Pub Date : 2020-01-01 , DOI: 10.1080/09540105.2020.1726879
Meiguo Xin 1 , Lin Zeng 1 , Di Ran 1 , Xiangmei Chen 1 , Yang Xu 2 , Daoxuan Shi 2 , Yonghong He 2 , Suyi Zhong 2
Affiliation  

ABSTRACT A label-free and rapid detection of cooked rat meat-based molecular-imprinted polymer (MIP) conjugated with quantum weak measurement system (QWM) has been developed. Cooked rat protein-specific MIP was prepared by a modified reverse-phase microemulsion method and coated on the internal surface of measuring channels assembled into Mach-Zrhnder interferometer of an optical weak measurement system. The measurement is based on the optical phase shift due to the refractive index change, which is induced by the specific capture of rat protein at its MIP sites. The results can be characterized and calculated by the central wavelength shift of output spectra through weak value amplification. This MIP-WQM system was demonstrated with cooked rat protein showing linear dynamic range from 0 to 20 µg/L. The limit of detection is calculated as 0.1 µg/L for cooked rat meat protein. The system also showed high specificity to cooked rat meat protein, compared to cooked pork meat proteins. GRAPHICAL ABSTRACT

中文翻译:

使用 MIP 量子弱测量对熟肉进行无标记快速识别

摘要开发了一种与量子弱测量系统(QWM)结合的熟大鼠肉基分子印迹聚合物(MIP)的无标记快速检测方法。采用改良的反相微乳液法制备煮熟的大鼠蛋白质特异性MIP,并涂覆在组装成光学弱测量系统的Mach-Zrhnder干涉仪的测量通道的内表面。该测量基于折射率变化引起的光学相移,这是由在其 MIP 位点特定捕获大鼠蛋白质引起的。结果可以通过弱值放大输出光谱的中心波长偏移来表征和计算。该 MIP-WQM 系统用煮熟的大鼠蛋白质进行了演示,显示出 0 到 20 µg/L 的线性动态范围。检测限计算为 0。熟大鼠肉蛋白质 1 µg/L。与煮熟的猪肉蛋白质相比,该系统还对煮熟的大鼠肉蛋白质显示出高度的特异性。图形概要
更新日期:2020-01-01
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