ABSTRACT In the current study, a rapid and novel biomimetic nano-enzyme-linked immunosorbent assay has been established for the detection of sulfadiazine (SDZ) using Au@SiO2 nanoparticles labelling as a marker. In this system, gold nanoparticles exhibited intrinsic peroxidase and activity with excellent water-solubility, and the SiO2 nanoparticles as ideal carries possessed a large surface area and ease of surface functionalization. The molecularly imprinted polymer films were selected as bionic antibody because of its advantages of selective recognition and reusability. Combine the above advantages, the method exhibited good stability, recognition capability and sensitivity. Under the optimal condition, the limit of detection of the immunosorbent assay was 0.2 mg/L. Beef sample spiked with SDZ was analysed, and the recoveries were in the 78.00% to 90.96% range. The assay could be used to detect SDZ residues in pork and chicken samples, and the results were not significantly different from those obtained by high-performance liquid chromatography.

中文翻译：

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基于Au@SiO2纳米酶标记的磺胺嘧啶仿生酶联免疫吸附测定新方法的开发

摘要 在目前的研究中，使用 Au@SiO2 纳米颗粒标记作为标记物，建立了一种快速且新颖的仿生纳米酶联免疫吸附测定法，用于检测磺胺嘧啶 (SDZ)。在该体系中，金纳米粒子表现出内在的过氧化物酶和活性，具有优异的水溶性，而作为理想载体的 SiO2 纳米粒子具有较大的表面积和易于表面功能化。分子印迹聚合物薄膜因其选择性识别和可重复使用的优点而被选为仿生抗体。综合以上优点，该方法表现出良好的稳定性、识别能力和灵敏度。在最佳条件下，免疫吸附试验的检测限为0.2 mg/L。对添加 SDZ 的牛肉样品进行了分析，回收率在 78 以内。00% 至 90.96% 范围。该测定法可用于检测猪肉和鸡肉样品中的SDZ残留，结果与高效液相色谱法获得的结果无显着差异。