ABSTRACT In a previous study, we developed a dihydropteroate synthase (DHPS)-based biosensor for multi-sulfonamides (SAs), which could detect 29 SAs with IC50 values below 100 ng mL−1. The purpose of the present study was to apply the DHPS-based biosensor to different food matrices and evaluate the influence of matrix effects on the performance of the biosensor by using sulfamethazine (SMZ) as a reference analyte. The results show that different sample pretreatment procedures were required for each food sample to ensure the accuracy and precision of the biosensor. The limit of detection (LOD) for SMZ in chicken, pork, egg and honey was 17.82, 20.55, 23.22, and 5.57 μg kg−1, respectively. The recovery values ranged from 47.6% to 135.1%, and the coefficients of variation (CV) were less than 25.6%. Taken together, our findings show that the DHPS-DHPPP biosensor is a suitable screening method for the screening of multi-SAs in different food matrices.

中文翻译：

---

通过基于二氢蝶酸合酶的生物传感器评估不同食物基质以筛选磺酰胺残留

摘要 在之前的一项研究中，我们开发了一种基于二氢蝶酸合酶 (DHPS) 的多磺酰胺 (SA) 生物传感器，它可以检测 IC50 值低于 100 ng mL-1 的 29 种 SA。本研究的目的是将基于 DHPS 的生物传感器应用于不同的食品基质，并通过使用磺胺二甲嘧啶 (SMZ) 作为参考分析物来评估基质效应对生物传感器性能的影响。结果表明，每个食品样品需要不同的样品预处理程序，以确保生物传感器的准确性和精密度。鸡肉、猪肉、鸡蛋和蜂蜜中 SMZ 的检测限 (LOD) 分别为 17.82、20.55、23.22 和 5.57 μg kg-1。回收率范围为 47.6% 至 135.1%，变异系数 (CV) 小于 25.6%。综合起来，