ABSTRACT Polysaccharide from Gynostemma pentaphyllum Makino (GPP) could prevent H22 tumour growth and have immunostimulatory in mice, but the mechanism is largely unknown. In this study, we further investigated whether GPP prevents tumour growth by inducing cytotoxicity to the tumour cells or enhancing immunity in H22 ascites tumour-bearing mice. The results showed that GPP prevented the H22 ascites tumour growth in vivo, but showed little effect on cell viability and cycles to H22 cells in vitro. GPP improved the proportion and mitochondrial level of T cells, and promoted the secretion of IL-2 and IFN-γ in the ascites of the mice. Furthermore, GPP in vitro enhanced the cell viability and promoted activation (in particular T cells), proliferation, and the secretion of IL-2 and IFN-γ of lymphocyte. Taken together, our results demonstrated that GPP prevents H22 ascites tumour growth by enhancing immunity rather than cytotoxicity in mice.

中文翻译：

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绞股蓝多糖通过增强免疫而不是细胞毒性来防止 h22 腹水肿瘤的生长

摘要绞股蓝牧野多糖（GPP）可以阻止H22肿瘤的生长并在小鼠体内具有免疫刺激作用，但其机制尚不清楚。在这项研究中，我们进一步研究了 GPP 是否通过诱导对肿瘤细胞的细胞毒性或增强 H22 腹水荷瘤小鼠的免疫力来阻止肿瘤生长。结果表明，GPP在体内阻止了H22腹水肿瘤的生长，但在体外对细胞活力和向H22细胞的循环几乎没有影响。GPP提高了T细胞的比例和线粒体水平，促进了小鼠腹水中IL-2和IFN-γ的分泌。此外，GPP 体外 增强了细胞活力并促进了淋巴细胞的活化（特别是T细胞），增殖和IL-2和IFN-γ的分泌。综合起来，