Development of novel C-nucleoside analogues for the formation of antiparallel-type triplex DNA with duplex DNA that includes TA and dUA base pairs,Organic & Biomolecular Chemistry

当前位置： X-MOL 学术 › Org. Biomol. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of novel C-nucleoside analogues for the formation of antiparallel-type triplex DNA with duplex DNA that includes TA and dUA base pairs
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2020-03-19 , DOI: 10.1039/d0ob00420k
Yosuke Taniguchi _{1,

2,

3,

4} , Yuya Magata _{1,

2,

3,

4} , Takayuki Osuki _{1,

2,

3,

4} , Ryotaro Notomi _{1,

2,

3,

4} , Lei Wang _{1,

2,

3,

4} , Hidenori Okamura _{1,

2,

3,

4} , Shigeki Sasaki _{1,

2,

3,

4}

Affiliation

Expansion of the triplex DNA forming sequence is required in the genomic targeting fields. Basically, triplex DNA is formed by the interaction between the triplex-forming oligonucleotides and homo-purine region with the target duplex DNA. The presence of the base pair conversion sites hampers stable triplex formation. To overcome this limitation, it is necessary to develop an artificial nucleic acid to recognize the base conversion sites, and the CG and TA base pairs. We describe the synthesis of C-nucleoside analogues and an evaluation of the ability of triplex formation. Consequently, the combined use of the novel C-nucleoside analogues, AY – AY-d(Y-NH₂), AY-d(Y-Cl) and ^IAP-d(Y-Cl), is capable of recognizing duplex DNA including the TA or dUA base pair.

中文翻译：

新型C-核苷类似物的开发，用于与包含TA和dUA碱基对的双链DNA形成反平行型三链DNA

在基因组靶向领域中需要扩大三链DNA形成序列。基本上，三链体DNA是通过三链体形成寡核苷酸和高嘌呤区与靶双链体DNA之间的相互作用形成的。碱基对转化位点的存在妨碍稳定的三链体形成。为了克服该限制，必须开发一种人工核酸以识别碱基转化位点以及CG和TA碱基对。我们描述了C-核苷类似物的合成和三链体形成能力的评估。因此，组合使用新颖C-核苷类似物，AY - AY-d（Y-NH ₂），AY-d（Y-C1）和^我AP-d（Y-CL），能够识别双链DNA的包括TA或dUA碱基对。

更新日期：2020-04-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11