To investigate the role of serum cytokine assays to distinguish between active from treated syphilis among serofast patients, we recruited individuals into a prospective cohort study. Participants underwent routine syphilis screening. We selected specimens from a majority cohort of serofast participants with treated and active syphilis. We analyzed specimens with a 62-cytokine multiplex bead-based enzyme-linked immunosorbent assay. Cytokines, BDNF and TNFB, were most predictive. We built a decision tree that was 82.4% accurate, 100% (95%CI: 82%-100%) sensitive, and 45% (95%CI, 18%-75%) specific. Our decision tree differentiated between serum specimens of serofast participants with treated syphilis and active syphilis.

中文翻译：

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细胞因子表达分析在区分活性与先前治疗的梅毒中的应用。

为了研究血清细胞因子检测在血清反应快患者中区分梅毒和梅毒的作用，我们招募了一组前瞻性队列研究。参加者接受常规梅毒筛查。我们从具有治疗性和活动性梅毒的大多数血清快速参与者中选择了标本。我们用基于62细胞因子多重珠的酶联免疫吸附试验分析了标本。细胞因子，BDNF和TNFB是最可预测的。我们构建的决策树的准确度为82.4％，敏感度为100％（95％CI：82％-100％），特定度为45％（95％CI，18％-75％）。我们的决策树区分了治疗过的梅毒和活动性梅毒的血清快速参与者的血清标本。