Optofluidic biolaser is an emerging technology for chemical and biomedical sensing. However, the sensitivity of optofluidic laser is still limited by the laser threshold condition. In this paper we report a DC-biased optofluidic biolaser for uric acid (UA) detection. Inside a Fabry-Perot laser cavity, a coupled enzyme reaction was employed to transform the detection of UA into the detection of analyte-converted hydrogen peroxide (H2O2). The fluorescent product of the enzyme-catalyzed reaction, resorufin, was employed as gain medium for optofluidic lasing. An optimized concentration of H2O2 was pre-added as a DC bias to counterbalance the requirement of high analyte concentration for lasing. UA detection with a limit of detection of 3.63 μM was achieved with a low sample volume as small as 10 μl. This work provides a sensitive technology using optofluidic biolaser for the detection of substance in human body fluids that can be converted into H2O2.

中文翻译：

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用于尿酸检测的直流偏置光流体生物激光器

光流控生物激光器是一种用于化学和生物医学传感的新兴技术。然而，光流体激光的灵敏度仍然受到激光阈值条件的限制。在本文中，我们报告了一种用于尿酸 (UA) 检测的直流偏置光流控生物激光器。在 Fabry-Perot 激光腔内，采用耦合酶反应将 UA 的检测转化为分析物转化的过氧化氢 (H2O2) 的检测。酶催化反应的荧光产物试卤灵用作光流体激光的增益介质。预先添加了优化浓度的 H2O2 作为直流偏置，以抵消激光产生高分析物浓度的要求。UA 检测限为 3.63 μM，样品体积小至 10 μl。