A system containing spectral-domain phase microscopy and fluorescence microscopy has been developed to decode and detect thickness-encoded suspension arrays. The spectral-domain phase imaging path is used for decoding the thickness of the micro glass pieces while the fluorescence imaging path is used for quantifying the targeted analytes. The spectral-domain phase microscopy uses a spectral domain phase retrieval technique so that it has a high-dynamic thickness imaging range and submicron-scale thickness resolution, increasing the encoding efficiency and decoding precision. The thickness decoding capability of the system has been demonstrated by multiplexed immunoassay experiments. The quantitative analysis capability has been verified by concentration gradient experiments.

中文翻译：

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厚度编码悬浮阵列的谱域相位显微镜

已经开发出一种包含光谱域相位显微镜和荧光显微镜的系统来解码和检测厚度编码的悬浮阵列。光谱域相位成像路径用于解码微玻璃片的厚度，而荧光成像路径用于量化目标分析物。谱域相位显微技术采用谱域相位检索技术，具有高动态厚度成像范围和亚微米级厚度分辨率，提高了编码效率和解码精度。该系统的厚度解码能力已通过多重免疫测定实验证明。定量分析能力已通过浓度梯度实验验证。