Introduction

Endothelial cells cover the surface of the capillary wall and literature review has cemented its angiogenic roles in wound healing and tissue regeneration. However, the angiogenic in vitro models available are inadequate to understand the endothelial differentiation process.

Methods

A construct was made using human amniotic membrane (HAM) as a matrix to assist the dental stem cells to differentiate into endothelial-like cells. This study aimed to assess the biological interaction between stem cells from human exfoliated deciduous teeth (SHED) and the stromal side (SS) of the glycerol-preserved HAM in angiogenic-induced environment media using VEGF. The changes were evaluated through cell morphology, migration, as well as gene expression level.

Results

There were morphological changes observed in SHED in angiogenic-induced media. SHED appeared to be differentiated from fibroblast-like cells to a new structure, mimicking endothelial-like structure through microscopy analysis. Besides, the cross-section of the construct revealed that the cells seeded on the matrix were able to maintain its monolayer structure at day 1, 7 and 14 but infiltrated into the HAM at day 21, suggesting cell migration. The cells were also able to maintain its stemness (Nestin, Nanog and CD29) and at the same time express the angiogenic markers (IL-8, VEGF and MMP-2).

Conclusion

HAM promotes SHED proliferation, migration and has the potential as a differentiating matrix for endothelial-like cells.

中文翻译：

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人羊膜作为VEGF处理的牙科干细胞内皮分化的基质。

介绍

内皮细胞覆盖在毛细血管壁的表面，文献综述巩固了其在伤口愈合和组织再生中的血管生成作用。然而，可用的血管生成体外模型不足以了解内皮分化过程。

方法

使用人羊膜（HAM）作为基质来构建构建体，以协助牙齿干细胞分化为内皮样细胞。这项研究旨在评估使用血管内皮生长因子诱导的环境培养基中人脱落乳牙（SHED）的干细胞与甘油保存的HAM的基质侧（SS）之间的生物学相互作用。通过细胞形态，迁移以及基因表达水平评估变化。

结果

SHED在血管生成诱导的培养基中观察到形态学变化。SHED似乎从成纤维细胞样细胞分化为一种新结构，通过显微镜分析模仿了内皮样结构。此外，构建体的横截面表明，接种在基质上的细胞能够在第1、7和14天维持其单层结构，但在第21天渗入HAM，表明细胞迁移。这些细胞还能够维持其干性（巢蛋白，Nanog和CD29），同时表达血管生成标记（IL-8，VEGF和MMP-2）。

结论

HAM促进SHED增殖，迁移，并具有作为内皮样细胞分化基质的潜力。