Thermophilic anaerobic digestion (TAD) is an efficient method for biogas production. In this study, the TAD of Arundo donax cv. Lvzhou No. 1 (ADL-1, a new kind of energy crop with high cold tolerance) at different growth stages was carried out, in order to investigate the relationship between microbial community structure and its function during the fermentation process. The results showed that the most optimal growth period of ADL-1 was 3 months, regarding the yield of biogas production. The TAD process lasted for 10 days with cumulative biogas and methane yields of 312.7 mL/g VS and 231 mL/g VS, respectively. The degradation rates of hemicellulose, cellulose, and lignin were 41.78%, 27.99%, and 14.46%, respectively. The high-throughput sequencing of 16S rRNA gene amplicons revealed that the most abundant bacterial phylum in TAD was Firmicutes with three dominant genera of Tepidiphilus, Sedimentibacter, and Gelria. Also, the main archaeal order was Methanomicrobiales, in which Methanoculleus and Methanosarcina were detected as dominant genera. Therefore, this article reveals the dynamic changes of structure and function of microbial communities during TAD of ADL-1, providing the theoretical basis for the development of energy crops with cold tolerance as potential biogas feedstock.

中文翻译：

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Arundo donax cv的嗜热厌氧消化。绿洲第一沼气生产：微生物群落的结构和功能分析

高温厌氧消化（TAD）是生产沼气的有效方法。在这项研究中，Arundo donax的TAD简历。为了研究发酵过程中微生物群落结构与其功能的关系，研究了绿洲1号（ADL-1，一种耐寒性高的新型能源作物）。结果表明，就沼气产量而言，ADL-1的最佳生长期为3个月。TAD过程持续了10天，累计沼气和甲烷产量分别为312.7 mL / g VS和231 mL / gVS。半纤维素，纤维素和木质素的降解率分别为41.78％，27.99％和14.46％。高通量的16S rRNA基因扩增子测序揭示了在TAD中最丰富的细菌门是厚壁菌门有三个优势属Tepidiphilus，Sedimentibacter和Gelria。此外，主古细菌顺序为Methanomicrobiales，其中Methanoculleus和甲烷被检测为优势属。因此，本文揭示了ADL-1在TAD期间微生物群落结构和功能的动态变化，为开发具有耐寒性的能源作物作为潜在沼气原料提供了理论依据。