A consolidated bioprocessing (CBP) approach utilizing a synergistic co-culture of three lignocellulolytic bacteria (Clostridium thermocellum, C. stercorarium, and Thermoanaerobacter thermohydrosulfuricus) was compared with the monoculture controls using a variety of different lignocellulosic substrates. In addition to increasing the average ethanol:acetate:lactate ratio from 49:45:6 in the C. thermocellum monoculture to 66:26:7 in the tri-culture, the amount of total polysaccharides utilized in the tri-culture relative to the monocultures was increased in all untreated and pretreated substrates tested, with up to 3-fold increases observed. Under relatively low carbon loads of 2 g/L, the highest ethanol titre achieved was 0.298 g/L, corresponding to a yield of 0.149 g ethanol/g substrate. The co-culture was then used to screen the digestibility of different cattail (Typha spp.) species harvested in different seasons as well as wheat straw samples from different cultivars that vary in their resistance to lodging. Cattail plants that harvested in July contained less cellulose and lignin and were up to 80% more digestible than those harvested in September. Although moderate differences in wheat straw digestibility were observed, this variation did not significantly correlate with differences in lodging resistance or cellulose, hemicellulose, and lignin contents. Our study suggests the importance of screening feedstocks using CBP approaches in order to determine the attributes that affect digestibility with CBP.

中文翻译：

---

使用嗜热厌氧菌的共培养物进行合并生物加工的小麦和香蒲生物量的消化率

采用三个木质纤维素菌（的协同共培养甲联合生物加工（CBP）的方法热纤维梭菌，Ç。粪堆，和嗜热thermohydrosulfuricus）使用各种不同的木质纤维素底物的单作对照进行了比较。除了增加的平均乙醇：在6：从49乳酸比：：乙酸盐45 Ç。热纤维在三元培养物中将单培养培养物的比例提高到66：26：7，在所有未处理和预处理的底物中，相对于单培养物，在三培养物中使用的总多糖量都增加了，观察到的增长高达3倍。在2 g / L的相对低碳负荷下，获得的最高乙醇滴定度为0.298 g / L，对应于0.149 g乙醇/ g底物的产量。然后使用共培养物筛选不同香蒲（香蒲）的消化率。spp。）物种在不同季节收获，以及来自不同品种的小麦秸秆样品，其抗倒伏性各不相同。7月收获的香蒲植物所含纤维素和木质素含量比9月收获的植物少，其消化率高达80％。尽管观察到小麦秸秆消化率存在中等差异，但这种差异与抗倒伏性或纤维素，半纤维素和木质素含量的差异没有显着相关。我们的研究表明使用CBP方法筛选原料的重要性，以确定影响CBP消化率的属性。