Two culture media were tested for the production of bacterial nanocellulose (BNC) under static culture fermentation, one containing molasses (Mol-HS), the other molasses and corn steep liquor (Mol-CSL), as a source of carbon and nitrogen, respectively. These are low-cost nutrients widely available, which provide very good BNC productivities. However, the use of these substrates generates wastewaters with high organic loads. Anaerobic digestion is one of the most promising treatments for industrial wastewaters with high organic loads since, beyond removal of the organic matter, it generates energy, in form of biogas. The wastewaters from BNC fermentation were thus evaluated for their biochemical methane potential through anaerobic digestion. For this, two wastewaters streams were collected: (i) the culture medium obtained after fermentation (WaF) and (ii) the WaF combined with BNC washing wastewaters (WaW). These two effluents—WaF and WaW—were characterized regarding their chemical oxygen demand, total nitrogen, total and volatile solids, to assess their suitability for anaerobic digestion. The biochemical methane potential of WaF and WaW from Mol-CSL wastewaters was (387 ± 14 L kg⁻¹ VS) and (354 ± 4 L kg⁻¹ VS), corresponding to a methanization percentage of (86.9 ± 3.1) % and (79.5 ± 0.9) %, respectively. After treatment, the chemical oxygen demand of WaF and WaW was reduced by (89.2 ± 0.4) and (88.7 ± 1.5), respectively. An exploratory test using an Upflow Anaerobic Sludge Blanket reactor for WaW treatment was also performed. The reactor was operated with a organic loading rate of [(6.5 ± 0.1) g L⁻¹ d⁻¹] and hydraulic retention time of 3.33 days, allowing a chemical oxygen demand removal of 58% of WaW. Results here obtained demonstrate, for the first time, the high potential of AD for the valorisation of the BNC fermentation wastewaters.

中文翻译：

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对细菌纳米纤维素生产中产生的废水进行研究和评估。

测试了两种培养基在静态培养发酵下产生细菌纳米纤维素（BNC）的能力，一种培养基分别含有糖蜜（Mol-HS），另一种糖蜜和玉米浆（Mol-CSL）作为碳和氮的来源。 。这些是广泛可用的低成本营养素，可提供非常好的BNC生产率。但是，使用这些基材会产生有机负荷较高的废水。厌氧消化是具有高有机负荷的工业废水最有希望的处理方法之一，因为除去除有机物外，它还以沼气的形式产生能量。因此，通过厌氧消化评估了BNC发酵产生的废水的生化甲烷潜力。为此，收集了两个废水流：（i）发酵后获得的培养基（WaF）和（ii）WaF与BNC洗涤废水（WaW）结合。对这两种废水（WaF和WaW）进行了化学需氧量，总氮，总固体和挥发性固体的表征，以评估它们是否适合厌氧消化。来自Mol-CSL废水的WaF和WaW的生化甲烷潜力为（387±14 L kg^-1 VS）和（354±4 L kg ^-1 VS），分别对应于（86.9±3.1）％和（79.5±0.9）％的甲烷化率。处理后，WaF和WaW的化学需氧量分别降低了（89.2±0.4）和（88.7±1.5）。还使用上流厌氧污泥毯反应器进行了WaW处理的探索性测试。反应器以[（6.5±0.1）g L ^-1  d ^-1 ]的有机负载率和3.33天的水力停留时间运行，从而允许去除58％WaW的化学需氧量。此处获得的结果首次证明了AD在BNC发酵废水的增值中具有很高的潜力。