Antimicrobial activity of silver nanoparticles (AgNPs) has been well documented in earlier studies. As their efficient role in combating phytopathogens has begun recently, there is a huge scope to explore their effectiveness in agriculture. Considering the strong antifungal activity of biosynthesized AgNPs (as reported in our previous study), our main aim is to elucidate their antibacterial activity against bacterial plant pathogens to authenticate their wide range of agricultural applications. The present manuscript highlights the potential role of biosynthesized AgNPs against Xanthomonas oryzae pv. oryzae (Xoo) causing disastrous sheath blight disease of rice worldwide. We observed strong antibacterial activity of biosynthesized AgNPs (size ~ 12 nm) against Xoo at 20, 30 and 50 µg/mL concentrations. The significant inhibitory impact of AgNPs on biofilm formation by Xoo was noted even at the lower dose of 5 µg/mL (p = 0.001). Maximum biofilm inhibition (p = 0.000) was caused at 50 µg/mL concentration of AgNPs in comparison to control. Furthermore, disease suppression by biosynthesized AgNPs was authenticated under greenhouse conditions. Foliar spray of AgNPs significantly reduced the blight symptoms in rice sheaths as shown by 9.25% DLA (% Diseased leaf area) as compared to 33.91% DLA in Xoo inoculated rice plants. Altogether, our data suggest that biosynthesized AgNPs based nanoformulation can be applied for successful management of blight disease of rice. In addition, the antibiofilm strategies instigated by AgNPs can be exploited against a wide range of bacterial phytopathogens. In light of rapidly emerging antibiotic-resistant microbial strains, the current work provides an alternate effective platform for the application of nanoformulation for augmenting sustainability in the agriculture.

中文翻译：

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生物制造的银纳米粒子对米黄单胞菌 pv. 的抗菌和生物膜抑制活性。引起水稻枯萎病的稻瘟病促进病害抑制

银纳米粒子 (AgNPs) 的抗菌活性在早期的研究中已有详细记载。由于它们在对抗植物病原体方面的有效作用最近开始发挥作用，因此有很大的空间可以探索它们在农业中的有效性。考虑到生物合成的 AgNPs 具有很强的抗真菌活性（如我们之前的研究中所报道），我们的主要目的是阐明它们对细菌植物病原体的抗菌活性，以验证其广泛的农业应用。本手稿强调了生物合成的 AgNPs 对 Xanthomonas oryzae pv 的潜在作用。oryzae (Xoo) 在世界范围内引起灾难性的水稻纹枯病。我们观察到生物合成的 AgNPs（尺寸 ~ 12 nm）在 20、30 和 50 µg/mL 浓度下对 Xoo 具有很强的抗菌活性。即使在 5 µg/mL (p = 0.001) 的较低剂量下，也注意到 AgNPs 对 Xoo 生物膜形成的显着抑制影响。与对照相比，最大生物膜抑制 (p = 0.000) 是在 50 µg/mL 浓度的 AgNPs 下引起的。此外，生物合成的 AgNPs 对疾病的抑制在温室条件下得到了验证。与 Xoo 接种的水稻植株中的 33.91% DLA 相比，AgNPs 的叶面喷洒显着减少了稻鞘中的枯萎病症状，如 9.25% DLA（患病叶面积百分比）所示。总而言之，我们的数据表明基于生物合成的 AgNPs 纳米制剂可用于成功管理水稻枯萎病。此外，AgNPs 引发的抗生物膜策略可用于对抗广泛的细菌植物病原体。