Cerebrovascular accident (CVA) is one of the leading causes of death and disability worldwide, as well as a major financial burden for health care systems. CVA rodent models provide experimental support to determine possible in vivo therapies to reduce brain injury and consequent sequelae. This study analyzed nociceptive, motor, cognitive and mood functions in mice submitted to distal middle cerebral artery (DMCA) occlusion. Male C57BL mice (n = 8) were randomly allocated to control or DMCA groups. Motor function was evaluated with the tests: grip force, rotarod and open field; and nociceptive threshold with von Frey and hot plate assessments. Cognitive function was evaluated with the inhibitory avoidance test, and mood with the tail suspension test. Evaluations were conducted on the seventh- and twenty-eighth-day post DMCA occlusion to assess medium- and long-term effects of the injury, respectively. DMCA occlusion significantly decreases muscle strength and spontaneous locomotion (p < 0.05) both medium- and long term; as well as increases immobility in the tail-suspension test (p < 0.05), suggesting a depressive-type behavior. However, DMCA occlusion did not affect nociceptive threshold nor cognitive functions (p > 0.05). These results suggest that, medium- and long-term effects of DMCA occlusion include motor function impairments, but no sensory dysfunction. Additionally, the injury affected mood but did not hinder cognitive function.

中文翻译：

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实验性局灶性缺血性中风小鼠模型的中期和长期功能行为评估。

脑血管意外 (CVA) 是全球死亡和残疾的主要原因之一，也是医疗保健系统的主要经济负担。CVA 啮齿动物模型提供实验支持，以确定可能的体内疗法，以减少脑损伤和随之而来的后遗症。本研究分析了大脑中动脉远端 (DMCA) 闭塞小鼠的伤害感受、运动、认知和情绪功能。雄性 C57BL 小鼠（n = 8）被随机分配到对照组或 DMCA 组。运动功能通过以下测试进行评估：握力、旋转杆和开放场；和痛觉阈值与冯弗雷和热板评估。认知功能通过抑制性回避测试进行评估，情绪通过悬尾测试进行评估。对 DMCA 闭塞后第 7 天和第 28 天进行评估，分别评估损伤的中期和长期影响。DMCA 阻塞显着降低肌肉力量和自发运动（p  < 0.05) 中长期；以及在尾悬测试中增加不动（p  < 0.05），表明抑郁型行为。然而，DMCA 阻塞不影响伤害性阈值和认知功能（p  > 0.05）。这些结果表明，DMCA 阻塞的中长期影响包括运动功能障碍，但没有感觉功能障碍。此外，损伤会影响情绪，但不会影响认知功能。