A comparative experimental analysis by EPR spectroscopy of the intensity of nitric oxide (NO) production and the content of the copper in the tissues of olfactory bulb of the brain of male Wistar rats were performed after modeling of ischemic stroke and treatment with immediate intranasal injection of mesenchymal stem cells (MSCs). Brain ischemia was simulated by ligation at the level of bifurcation of the common carotid arteries. It was found a significant reduction of NO content in the olfactory bulb of the brain of rats on the 1st and 2nd days after modeling of ischemia. The level of NO production was also reduced on the 1st and 2nd days after ischemia with MSCs’ administration as compared to intact animals. It was not found the significant difference of the NO content in rats after ischemia with MSCs’ administration relative to ischemic rats. The copper content in the olfactory bulb of the rat, which corresponds to the level of superoxide dismutase 1 and 3, tended to increase after modeling ischemia and remained for 2 days. The MSCs’ administration was accompanied by a significant increase in copper content on the 1st day after modeling of ischemia and by decrease of its content on 2nd day after ischemia. The experiments showed that MSCs’ administration did not affect the intensity of NO production on the 1st and 2nd days after the modeling of brain ischemia, but was accompanied by an increase in the antioxidant protection of the nervous tissue 1 day after ischemia.

中文翻译：

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脑卒中建模和间充质干细胞鼻内给药后大鼠大脑嗅球中一氧化氮和铜含量的变化

采用EPR光谱法对雄性Wistar大鼠大脑嗅球组织中一氧化氮（NO）产生强度和铜含量进行对比实验分析，在缺血性脑卒中建模和即刻鼻腔注射治疗后进行了对比实验。间充质干细胞 (MSC)。通过在颈总动脉分叉处结扎来模拟脑缺血。缺血造模后第1天和第2天，大鼠大脑嗅球中NO含量显着降低。与完整动物相比，在缺血后第 1 天和第 2 天，施用 MSCs 的 NO 产生水平也降低。与缺血大鼠相比，MSCs给药后大鼠缺血后NO含量未见显着差异。大鼠嗅球中的铜含量（对应于超氧化物歧化酶 1 和 3 的水平）在建模缺血后趋于增加并保持 2 天。MSCs 的给药伴随着缺血后第 1 天铜含量的显着增加和缺血后第 2 天铜含量的减少。实验表明，MSCs的给药不影响脑缺血造模后第1天和第2天NO产生的强度，但伴随着缺血1天后神经组织的抗氧化保护作用增加。MSCs 的给药伴随着缺血后第 1 天铜含量的显着增加和缺血后第 2 天铜含量的减少。实验表明，MSCs的给药不影响脑缺血造模后第1天和第2天NO产生的强度，但伴随着缺血1天后神经组织的抗氧化保护作用增加。MSCs 的给药伴随着缺血后第 1 天铜含量的显着增加和缺血后第 2 天铜含量的减少。实验表明，MSCs的给药不影响脑缺血造模后第1天和第2天NO产生的强度，但伴随着缺血1天后神经组织的抗氧化保护作用增加。