Abstract

This work is dedicated to proving our hypothesis that catecholamines and their metabolites play a crucial role in the development of retinopathy of prematurity, which leads to progressive uncontrollable vascularization in the retina, leading to blindness. The study was performed in an animal model of retinopathy of prematurity, which was achieved by hyperoxygenation in rats on postnatal days 7, 14, 21, and 30. The content of catecholamines and their metabolites in the retina of rats was determined by high performance liquid chromatography with electrochemical detection. It was shown that, in the rats with retinopathy, the content of L-DOPA on days 21 and 30 was decreased as compared to the control, whereas the content of noradrenaline on day 14 life increased compared to the control. However, we did not observe changes in the content of dopamine in the experimental animals relative to the control in any period studied. Given the published data on the involvement of catecholamines in the regulation of vasculogenesis in the retina in normal state, our data on the changes in the catecholamine metabolism in the retina in the model of retinopathy of prematurity can be regarded as evidence of the important role of catecholamines in the pathogenesis of this severe disease.

中文翻译：

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儿茶酚胺在大鼠早产儿视网膜病变实验模型中在病理性视网膜新血管形成发展中的作用。

摘要

这项工作致力于证明我们的假设，即儿茶酚胺及其代谢产物在早产儿视网膜病变的发展中起着至关重要的作用，这会导致视网膜进行性不可控制的血管形成，从而导致失明。该研究是在早产儿视网膜病变的动物模型中进行的，该模型通过在出生后第7、14、21和30天在大鼠中进行过氧化来实现。在大鼠视网膜中儿茶酚胺及其代谢产物的含量通过高效液相色谱法测定电化学检测色谱。结果表明，在视网膜病变大鼠中，与对照组相比，第21天和第30天的L-DOPA含量降低，而在第14天的寿命中去甲肾上腺素的含量却增加。然而，我们没有观察到在任何研究期间相对于对照，实验动物中多巴胺含量的变化。鉴于已发表的有关儿茶酚胺参与调节正常状态视网膜血管生成的数据，我们关于早产儿视网膜病变模型中视网膜儿茶酚胺代谢变化的数据可被认为是早产儿视网膜病变模型的重要证据。儿茶酚胺在这种严重疾病的发病机理中。