Abstract

A technique for the isolation and purification of ommochromes (pigments of the complex eye of the adult Hermetia illucens fly) has been developed. Isolated ommochromes showed fluorescence with emission maxima at 460–470 nm and 525–535 nm, the intensity of which increased abruptly during their oxidation with hydrogen peroxide. The method of chemiluminescence quenching of luminol was used to determine the antiradical activity of ommochromes; the constant value was (1.2 ± 0.5) × 10⁴ M^–1. A finely dispersed mixture of ommochromes in 0.1 M potassium phosphate buffer (pH 7.4) containing bovine serum albumin (2.0 mg/mL) showed antimicrobial activity against B. subtilis ATCC 6633, Candida albicans ATCC 2091, and Aspergillus niger INA 00760. The H. illucens imago was concluded to be a promising renewable source of natural pigments with good antioxidant characteristics and antibacterial properties.

中文翻译：

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Hermetia illucens的眼色素：抗氧化特性和抗菌活性的分离和研究

摘要

已经开发了用于分离和纯化全色素（成虫Hermetia illucens蝇的复眼色素）的技术。分离的全色素在460–470 nm和525–535 nm处显示最大发射荧光，其强度在被过氧化氢氧化时会突然增加。用鲁米诺化学发光猝灭法测定全色素的抗自由基活性。恒定值为（1.2±0.5）×10 ⁴ M ^–1。在含牛血清白蛋白（2.0 mg / mL）的0.1 M磷酸钾缓冲液（pH 7.4）中，全色素的精细分散混合物显示出对枯草芽孢杆菌ATCC 6633，白色念珠菌ATCC 2091和黑曲霉INA 00760.的H. illucens意象得出的结论是具有良好的抗氧化性能和抗菌性能的天然色素的前景的可再生来源。