Abstract

The biological properties, structural characterization, and performance of Klebsiella variicola GN02 isolated from the roots of P. sinense and embedded in sodium alginate supplemented with 10% sterile skim milk were investigated. The results showed that the immobilized K. variicola GN02 particles were more stable and had a higher survival rate than free bacteria. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis showed that immobilized K. variicola GN02 particles had the peaks associated with sodium alginate and K. variicola GN02 cells, indicating that K. variicola GN02 cells were successfully embedded in the sodium alginate. Compared with free bacteria, the immobilized K. variicola GN02 cells revealed slower but a longer growth period during the 40 h culture period. The OD₆₀₀ and ammonia secretion of immobilized K. variicola GN02 particles were 0.87 and 18.45 mg/L, respectively. A solution incubated with immobilized K. variicola GN02 exhibited a growth-promoting effect on corn than free K. variicola GN02. Root length, plant height, fresh weight, and total nitrogen content of plants subjected to immobilized K. variicola GN02 increased by 15.3, 16.3, 8.9, and 32.3%, respectively. The number of viable K. variicola GN02 in soil also increased significantly to 20.86 × 10⁵ CFU/g. Therefore, immobilized K. variicola GN02 has promising application value, and this study provides a theoretical basis and reference for developing a biological fertilizer with new nitrogen-fixing bacteria.

中文翻译：

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狼尾草根系固氮高效固定水痘克雷伯氏菌GN02细胞的制备及性质

摘要

研究了从中华假单胞菌根中分离并包埋在藻酸钠中补充10％无菌脱脂乳的水痘克雷伯氏菌GN02的生物学特性，结构特征和性能。结果表明，与游离细菌相比，固定化的水曲霉GN02颗粒更稳定，存活率更高。红外光谱，扫描电子显微镜和热重分析表明，固定的水曲霉GN02颗粒具有与藻酸钠和水曲霉GN02细胞相关的峰，表明水曲霉GN02细胞已成功嵌入海藻酸钠中。与游离细菌相比，固定化的水曲霉GN02细胞在40小时培养期间显示出较慢但较长的生长期。固定化的水曲霉GN02颗粒的OD ₆₀₀和氨分泌分别为0.87和18.45 mg / L。与固定的水曲霉GN02孵育的溶液比游离水曲霉GN02对玉米显示出促进生长的作用。固定化水曲霉GN02使植物的根长，株高，鲜重和总氮含量分别增加了15.3％，16.3、8.9和32.3％。可行的水曲霉数土壤中的GN02也显着增加至20.86×10 ⁵ CFU / g。因此，固定化水曲霉GN02具有广阔的应用价值，本研究为开发新型固氮菌生物肥料提供了理论依据和参考。