This research was developed to investigate the use of Azospirillum brasilense to enhance N use in wheat cropping systems and to assess the potential interactions between inoculation and urease inhibitor N-(n-butyl)thiophosphoric triamide (NBPT). The study was set up under a no-till system in a Rhodic Hapludox. The experimental design was a randomized completely block design with four replicates arranged in a two N source (urea with and without NBPT) × five N rate applied as side dress (0–200 kg ha⁻¹) × two inoculation (with and without A. brasilense) factorial scheme. Inoculation with A. brasilense in combination with application of 100 and 150 kg N ha⁻¹ was found to increase grain yield by 19.6 and 18.8%, respectively. Inoculation also increased biomass N uptake by 66.9% when N was omitted. Inoculation and NBPT application were found to increase NUE by 23.7% compared to inoculation in combination with conventional urea and by 140% compared to NBPT without inoculation. In addition, inoculation increased plant height and NUE by 13.2 and 49%, respectively, compared with non-inoculated plots. Conventional urea and urea with NBPT had a similar effect on nutrient concentration, N uptake, leaf chlorophyll index, biomass weight and wheat grain yield. This study showed positive improvements in wheat production parameters as a result of inoculation, but the potential benefits of NBPT use were less evident. Further research should be conducted under growing conditions that provide increased N volatilization to better study the potential of NBPT application.

中文翻译：

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NBPT脲酶抑制剂与巴西细螺旋藻的接种可以改善小麦的发育吗？

开展这项研究的目的是调查巴西固氮螺菌在小麦种植系统中的氮利用量，并评估接种与脲酶抑制剂N-（n-丁基）硫代磷酸三酰胺（NBPT）之间的潜在相互作用。该研究是在Rhodic Hapludox的免耕系统下进行的。实验设计是一个随机的完全区组设计，在两个氮源（有和没有NBPT的尿素）中安排了四个重复试验×施用了5 N的侧服（0–200 kg ha ^-1）×两次接种（有和没有A 。brasilense）析因方案。接种巴西青霉菌并结合施用100和150 kg N ha ^-1被发现分别提高了谷物产量19.6和18.8％。当省略氮时，接种也使生物量氮吸收增加了66.9％。发现与常规尿素联合接种相比，接种和NBPT施用可增加NUE 23.7％，与不接种NBPT相比，可增加NUE 140％。此外，与未接种地块相比，接种后植物株高和NUE分别增加了13.2％和49％。常规尿素和含NBPT的尿素对养分浓度，氮吸收，叶绿素指数，生物量和小麦籽粒产量具有相似的影响。这项研究表明，接种可提高小麦生产参数，但使用NBPT的潜在好处却不那么明显。