Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson’s disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People’s Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. atrata from the three other cicada species, and detected the adulteration of market CP samples. This SCAR assay is a rapid, simple, cheap, reliable, and reproducible method for species identification, regardless of sample form and status, and contributes to CP quality control.

中文翻译：

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基于COI的SCAR分析法快速简便地鉴定蝉蝉

蝉蝉（Cicadaida periostracum，CP），是蝉蝉的医学名称，是众所周知的昆虫衍生的传统药物，具有多种药理作用，例如抗惊厥，消炎，镇咳和抗癌作用；它也对帕金森氏病的治疗有益。对于适当的CP应用，准确的物种识别至关重要。韩国药典和中华人民共和国药典定义了隐隐隐孢子虫作为CP的唯一真实来源。但是，由于市场上许多蝉的组合包装，分布过程中的损坏以及加工成粉末形式，很难根据形态特征对商业分布的CP进行物种鉴定。基于DNA的分子标记是形态检测的绝佳替代方法。在这项研究中，线粒体细胞色素C氧化酶亚基I的序列C.蝉，Meimuna opalifera，蟪和Hyalessa maculaticollis进行分析。在序列比对的基础上，我们开发了序列特征化的扩增区域（SCAR）标记，可进行有效的物种鉴定。这些标记成功地鉴别了C. atrata从其他三个蝉物种中检出，并检测出市场CP样品的掺假。这种SCAR分析方法是一种快速，简单，便宜，可靠且可重现的物种鉴定方法，无论样品的形式和状态如何，都有助于CP质量控制。