Euphilotes ancilla purpura and cryptica (Lycaenidae), butterflies endemic to the Spring Mountains (Clark Co., Nevada), have been described as two univoltine, temporally isolated, sympatric taxa that utilize different early- and late-flowering larval host plant varieties (Eriogonum umbellatum). However, our results from field and laboratory indicate that this is not the case. The subspecies overlap in timing of adult reproductive flight (compilation of field records 1977 to 2018) and laboratory emergence of adults from early-season, non-diapause pupae indicate butterflies are not univoltine. Genetic samples collected from putative E. a. purpura (Early cohort) and cryptica (Late cohort) subpopulations show no evidence of genetic structure indicative of allochronic isolation in phylogenies of 26 mitochondrial DNA COI haplotypes and 18 nuclear ITS1 alleles. Analysis of molecular variance revealed 89% of mitochondrial DNA variation structured within and among subpopulations, with only 11% between the purportedly isolated subspecies. Analysis of isolation and migration indicated gene flow from the Early to Late cohort was 3 × greater than in the opposite direction. We conclude that, rather than two separate subspecies, Euphilotes ancilla exists in a network of partially interconnected subpopulations extending from 1750 to 3000 m across much of the Spring Mountains. Gene flow is related to the timing of adult flight and host plant flowering, contributing to the genetic variation in phenology necessary for evolutionary tracking of shifting flowering periods of larval host plants. Maintenance of connectivity and gene flow across the Spring Mountains is therefore essential for population persistence of both cohorts in the face of environmental change.

优灰蝶属附属物紫癜和隐秘（灰蝶科），蝴蝶特有的春山（克拉克公司，内华达州），已经被描述为两个一化性，暂时隔离，即利用不同的早期和晚花幼虫寄主植物品种的同域类群（Eriogonum umbellatum）。但是，我们从现场和实验室获得的结果表明情况并非如此。这些亚种在成年生殖飞行的时间上重叠（从1977年到2018年的实地记录汇编），并且成年早期从成年，无滞育pa的实验室出现表明蝴蝶不是单电压的。从推定的大肠杆菌中收集遗传样品。紫癜（早期队列）和隐ica（晚期队列）亚群没有显示表明26种线粒体DNA COI单倍型和18种核ITS1等位基因系统发育中等时分离的遗传结构证据。分子变异分析表明，在亚群内和亚群之间构造的线粒体DNA变异为89％，据称分离的亚种之间只有11％。分离和迁移的分析表明，从早期到晚期队列的基因流量比相反方向大3倍。我们得出的结论是，Euphilotes ancilla不是两个单独的亚种存在于部分连通的子种群网络中，这些子种群的范围从1750到3000 m，跨越了许多春山山脉。基因流与成年飞行和寄主植物开花的时间有关，这有助于物候期进化跟踪幼虫寄主植物的开花期所必需的物候方面的遗传变异。因此，面对环境变化，维持春季山区的连通性和基因流动对于这两个队列的种群持续生存至关重要。