Dark fermentation of food leftovers for hydrogen production has been studied using a two-stage upflow anaerobic biofilter reactor. To determine the optimum temperature for hydrogen production, the two reactors were operated in parallel at two different temperatures: 35 °C and 45 °C as suitable for prevailing ambient temperature in Egypt. The results indicated that hydrogen production at 45 °C was higher than that at 35 °C. To determine the optimum organic loading rate, a comparative study was carried out using two different OLRs: 10- and 20-g COD/L day. The system was run in a consecutive manner. The first bioreactor for H₂ production and the second for CH₄ production. Under optimal temperature (45 °C), the hydrogen production rates were 51.9 mL/L day and 10 mL/L day for OLRs 20- and 10-g COD/L day, respectively. When a drop in H₂ production occurred, in situ aeration for 24 h was carried out to deactivate methanogens. The total energy production was improved by combining H₂ and CH₄ bioreactors.

已经使用两级上流厌氧生物滤池反应器研究了剩余的食品中用于制氢的黑暗发酵。为了确定制氢的最佳温度，两个反应器在两个不同的温度下并行运行：35°C和45°C，适合埃及的主要环境温度。结果表明，在45℃下的氢气产生高于在35℃下的氢气产生。为了确定最佳有机负载率，使用两种不同的OLR：10 g和20 g COD / L天进行了比较研究。该系统以连续方式运行。第一个用于生产H _2的生物反应器，第二个用于CH _4的生物反应器生产。在最佳温度（45°C）下，对于20克和10克COD / L天的OLR，制氢速率分别为51.9 mL / L天和10 mL / L天。当H ₂产生量下降时，原位通气24 h使产甲烷菌失活。通过结合使用H ₂和CH ₄生物反应器，可以提高总的能量生产。