S. Enteritidis continues to be the most common pathogen of farm animals and a major public health burden worldwide. Using bacteriophages is a potential alternative to antibiotics against S. Enteritidis infection. In this study, the genome analysis of the lytic phage vB_SenM-PA13076 (PA13076) infecting S. Enteritidis revealed a linear, double-stranded DNA genome, which comprised of 52,474 bp and contained 69 ORFs. It belongs to the order Caudovirales; family Myoviridae, genus unclassified. The genes coded for DNA packaging, phage structural proteins, lysis components, DNA recombination, regulation, modification, and replication. No bacterial virulence or drug-resistance genes were detected. The phage PA13076 protected mice from a lethal dose of S. Enteritidis 13076^Amp (5 × 10⁸ CFU) by reducing the concentration of bacterial cells in blood, intestine, liver, spleen, and kidney. The phage PA13076 achieved at least 2.5 log reductions of S. Enteritidis cells in infected mice within 24 h (P < 0.05) when compared to the organs of control mice. The data also indicated that phage PA13076 could rapidly enter the blood and four organs of infected mice, remaining therein at concentrations of>10⁴ PFU/g for at least 72 h. These results show that phage PA13076 has definite potential as an antibacterial therapeutic agent for attenuating S. Enteritidis infections.

小号。肠炎仍然是农场动物最常见的病原体，也是全世界主要的公共卫生负担。使用噬菌体是针对抗生素的潜在替代物S.肠炎感染。在这项研究中，裂解噬菌体vB_SenM-PA13076（PA13076）感染S的基因组分析。肠炎沙门氏菌揭示了一个线性的双链DNA基因组，由52,474 bp组成，包含69个ORF。它属于Caudovirales秩序; 肌病毒科，属未分类。这些基因编码为DNA包装，噬菌体结构蛋白，裂解成分，DNA重组，调控，修饰和复制。未检测到细菌毒力或耐药基因。噬菌体PA13076从致命剂量的保护小鼠小号。通过降低血液，肠，肝，脾和肾中细菌细胞的浓度来提高肠炎肠炎13076^安培（5×10 ⁸ CFU）。噬菌体PA13076实现了S的至少2.5log的减少。与对照组小鼠的器官相比，感染小鼠的肠炎细胞在24小时内（P <0.05）。数据还表明，噬菌体PA13076可以迅速进入血液和受感染小鼠的四个器官，并以> 10 ^4的浓度保留在其中。PFU / g至少持续72小时。这些结果表明，噬菌体PA13076作为用于减毒S的抗菌治疗剂具有确定的潜力。肠炎感染。