Natural polysaccharides with versatile properties are the potential candidates for wound healing applications. In this study, an exopolysaccharide, EPS-S3, isolated from marine bacteria Pantoea sp. YU16-S3 was evaluated for its wound-healing abilities by studying the key molecular mechanisms in vitro and in vivo. Basic characterisation showed EPS-S3 as heteropolysaccharide with glucose, galactose, N-acetyl galactosamine and glucosamine. The molecular weight of EPS-S3 was estimated to be 1.75 × 10⁵ Da. It showed thermal stability up to 200 °C and shear-thickening non-Newtonian behaviour. It was biocompatible with dermal fibroblasts and keratinocytes and showed cell adhesion and cell proliferation properties. EPS-S3 facilitated cell migration in fibroblasts, induced rapid transition of cell cycle phases and also activated macrophages. In vivo experiments in rats showed the re-epithelialization of injured tissue with increased expression of HB-EGF, FGF, E-cadherin and β-catenin in EPS-S3 treatment. The results indicate that EPS-S3 modulates healing process through Wnt/β-catenin pathway due to its unique characteristics. In conclusion, EPS-S3 biosynthesized by the marine bacterium is a potential biomolecule for cutaneous wound healing applications.

具有多种用途的天然多糖是伤口愈合应用的潜在候选者。在这项研究中，从海洋细菌Pantoea sp。中分离出一种外多糖EPS-S3 。YU16-S3通过研究的关键分子机制对其伤口愈合的能力评价在体外和体内。基本表征显示EPS-S3为杂多糖，具有葡萄糖，半乳糖，N-乙酰半乳糖胺和葡萄糖胺。EPS-S3的分子量估计为1.75×10 ⁵大。它在高达200°C的温度下表现出热稳定性，并且具有剪切增稠的非牛顿性行为。它与真皮成纤维细胞和角质形成细胞具有生物相容性，并显示出细胞粘附和细胞增殖特性。EPS-S3促进成纤维细胞的细胞迁移，诱导细胞周期阶段的快速转变以及活化的巨噬细胞。大鼠体内实验显示，在EPS-S3处理中，HB-EGF，FGF，E-钙黏着蛋白和β-连环蛋白表达增加，使受伤的组织重新上皮化。结果表明，EPS-S3具有独特的特征，可通过Wnt /β-catenin途径调节愈合过程。总之，由海洋细菌生物合成的EPS-S3是用于皮肤伤口愈合应用的潜在生物分子。