Three undescribed lupane-type triterpenoids (1-3), three undescribed oleanane-type triterpenoids (4-6), and ten known pentacyclic triterpenoids (7-16) were isolated from the seeds of Peganum harmala L. (Zygophyllaceae). Their structures were elucidated using various spectroscopic methods (IR, HR-ESI-MS, 1D, and 2D NMR). All the triterpenoids were screened for anti-proliferative activity against HeLa, HepG2, and SGC-7901 cells using the MTT method. Except compounds 1, 2, and 13, all the other triterpenoids exhibited potent cytotoxic activities against tumour cells. Compounds 3, 6, and 15 inhibited the proliferation of HeLa cells in a dose-dependent manner, as observed by the colony formation assay. When HeLa cells were treated with different doses of compounds 3, 6, and 15, the cell nuclei changed shape to a crescent form and were condensed and fragmented, as observed by Hoechst 33258 staining. Additionally, these three triterpenoids induced the apoptosis in HeLa cells, which was detected by Western blot analysis.

中文翻译：

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来自 Peganumharmala L. 种子的具有抗增殖作用的三萜类化合物。

三种未描述的羽扇豆型三萜类化合物 (1-3)、三种未描述的齐墩果烷型三萜类化合物 (4-6) 和十种已知的五环三萜类化合物 (7-16) 从 Peganumharmala L. (Zygophyllaceae) 的种子中分离出来。使用各种光谱方法（IR、HR-ESI-MS、1D 和 2D NMR）阐明了它们的结构。使用 MTT 方法筛选所有三萜类化合物对 HeLa、HepG2 和 SGC-7901 细胞的抗增殖活性。除化合物 1、2 和 13 外，所有其他三萜类化合物均对肿瘤细胞显示出有效的细胞毒活性。如集落形成试验所观察到的，化合物 3、6 和 15 以剂量依赖性方式抑制 HeLa 细胞的增殖。当 HeLa 细胞用不同剂量的化合物 3、6 和 15 处理时，如Hoechst 33258染色所观察到的，细胞核形状变为新月形并且被凝聚和破碎。此外，这三种三萜类化合物诱导 HeLa 细胞凋亡，这通过蛋白质印迹分析检测到。