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Increased telomere length and mtDNA copy number induced by multi-walled carbon nanotube exposure in the workplace.
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.jhazmat.2020.122569 Manosij Ghosh 1 , Lisa Janssen 1 , Dries S Martens 2 , Deniz Öner 1 , Jelle Vlaanderen 3 , Anjoeka Pronk 4 , Eelco Kuijpers 4 , Roel Vermeulen 3 , Tim S Nawrot 2 , Lode Godderis 5 , Peter Hm Hoet 1
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.jhazmat.2020.122569 Manosij Ghosh 1 , Lisa Janssen 1 , Dries S Martens 2 , Deniz Öner 1 , Jelle Vlaanderen 3 , Anjoeka Pronk 4 , Eelco Kuijpers 4 , Roel Vermeulen 3 , Tim S Nawrot 2 , Lode Godderis 5 , Peter Hm Hoet 1
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Carbon nanotubes (CNTs) except MWCNT-7 have been classified as Group 3 ["Not classifiable as to its carcinogenicity to humans"] by the IARC. Despite considerable mechanistic evidence in vitro/in vivo, the classification highlights a general lack of data, especially among humans. In our previous study, we reported epigenetic changes in the MWCNT exposed workers. Here, we evaluated whether MWCNT can also cause alterations in aging related features including relative telomere length (TL) and/or mitochondrial copy number (mtDNAcn). Relative TL and mtDNAcn were measured on extracted DNA from peripheral blood from MWCNT exposed workers (N = 24) and non-exposed controls (N = 43) using a qPCR method. A higher mtDNAcn and longer TL were observed in MWCNT exposed workers when compared to controls. Independent of age, sex, smoking behavior, alcohol consumption and BMI, MWCNT-exposure was associated with an 18.30 % increase in blood TL (95 % CI: 7.15-30.62 %; p = 0.001) and 35.21 % increase in mtDNAcn (95 % CI: 19.12-53.46 %). Our results suggest that exposure to MWCNT can induce an increase in the mtDNAcn and TL; however, the mechanistic basis or consequence of such change requires further experimental studies.
中文翻译：
多壁碳纳米管在工作场所暴露引起的端粒长度增加和mtDNA拷贝数增加。
IARC将除MWCNT-7以外的碳纳米管（CNT）归类为第3组[“其对人类的致癌性无法分类”]。尽管在体外/体内有大量的机械证据，但是该分类突出表明普遍缺乏数据，尤其是在人类中。在我们以前的研究中，我们报告了MWCNT暴露工人的表观遗传变化。在这里，我们评估了MWCNT是否还会引起衰老相关特征的改变，包括相对端粒长度（TL）和/或线粒体拷贝数（mtDNAcn）。使用qPCR方法对从MWCNT暴露的工作人员（N = 24）和未暴露的对照（N = 43）的外周血中提取的DNA测量相对TL和mtDNAcn。与对照组相比，在MWCNT暴露的工人中观察到更高的mtDNAcn和更长的TL。与年龄，性别，吸烟行为无关，酒精消耗和BMI，MWCNT暴露与血液TL增加18.30％（95％CI：7.15-30.62％; p = 0.001）和mtDNAcn增加35.21％（95％CI：19.12-53.46％）有关。我们的研究结果表明，暴露于MWCNT可以引起mtDNAcn和TL的增加。但是，这种变化的机理基础或结果需要进一步的实验研究。
更新日期：2020-03-20
中文翻译：
多壁碳纳米管在工作场所暴露引起的端粒长度增加和mtDNA拷贝数增加。
IARC将除MWCNT-7以外的碳纳米管（CNT）归类为第3组[“其对人类的致癌性无法分类”]。尽管在体外/体内有大量的机械证据，但是该分类突出表明普遍缺乏数据，尤其是在人类中。在我们以前的研究中，我们报告了MWCNT暴露工人的表观遗传变化。在这里，我们评估了MWCNT是否还会引起衰老相关特征的改变，包括相对端粒长度（TL）和/或线粒体拷贝数（mtDNAcn）。使用qPCR方法对从MWCNT暴露的工作人员（N = 24）和未暴露的对照（N = 43）的外周血中提取的DNA测量相对TL和mtDNAcn。与对照组相比，在MWCNT暴露的工人中观察到更高的mtDNAcn和更长的TL。与年龄，性别，吸烟行为无关，酒精消耗和BMI，MWCNT暴露与血液TL增加18.30％（95％CI：7.15-30.62％; p = 0.001）和mtDNAcn增加35.21％（95％CI：19.12-53.46％）有关。我们的研究结果表明，暴露于MWCNT可以引起mtDNAcn和TL的增加。但是，这种变化的机理基础或结果需要进一步的实验研究。
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