In this study, we aimed to investigate the sanitizing effects of peracetic acid (PAA), and the underlying mechanism of its antimicrobial action against Morganella psychrotolerans. The exposure of M. psychrotolerans to 20 ppm PAA for 5 min led to its decrease below the detection level, indicating that PAA has a significant antibacterial effect against M. psychrotolerans in vitro. Moreover, the viable counts of M. psychrotolerans on saury (Cololabis saira) surface were showed a reduction of 1.40 and 2.23 log CFU/cm² upon treatment with 80 ppm PAA for 1 and 5 min, respectively. Further, PAA treatment delayed the growth of M. psychrotolerans on saury surface during storage at 4 °C. Next, the antimicrobial mechanism of PAA against M. psychrotolerans cells was investigated, and the damage to the cell membrane and cell surface upon PAA treatment was observed using scanning electron microscopy (SEM) and epifluorescence microscopy. The chromosomal DNA and the protein profiles after PAA treatment were also analyzed. Form our results, we hypothesized that the bactericidal effect of PAA treatment was mainly attributed to damage the bacterial cell membrane. These results indicate that PAA may be an efficient disinfectant against M. psychrotolerans and has applications in seafood processing and storage.

在这项研究中，我们旨在研究过氧乙酸（PAA）的消毒作用，以及其对嗜水摩根氏菌（Morganella psychrotolerans）的抗菌作用的潜在机制。的曝光M. psychrotolerans至20ppm PAA为导致其低于检测水平降低5分钟，这表明PAA具有针对一个显著抗菌作用M. psychrotolerans在体外。此外，用80 ppm PAA处理1分钟和5分钟后，秋刀鱼（秋刀鱼）表面上的M. rotrotolerans的活菌数分别降低了1.40和2.23 log CFU / cm ²。进一步地，PAA治疗延缓了精神支原体的生长。在4°C下储存期间保持在刀鱼表面上。接下来，研究了PAA对迷迭香支原体细胞的抗菌机理，并使用扫描电子显微镜（SEM）和落射荧光显微镜观察了PAA处理后对细胞膜和细胞表面的损伤。还分析了PAA处理后的染色体DNA和蛋白质谱。根据我们的结果，我们假设PAA处理的杀菌作用主要归因于细菌细胞膜的破坏。这些结果表明，PAA可能是一种有效的抗精神支原体的消毒剂，并已在海鲜加工和存储中应用。