This study was to develop a reliable and simple high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to detect paeoniflorin, geniposide, saikosaponin b2, liquiritin, paeonol and atractylenolide Ⅲ in beagle plasma and to study pharmacokinetic of paeoniflorin and geniposide after single-dose administration of Danzhi Xiaoyao Pill (DZXY). Chromatographic separation was performed using an Agilent C18 column, and multiple reaction monitoring (MRM) mode was used. A gradient elution procedure was used with solvent A (acetonitrile) and solvent B (0.1 % formic acid-water) as mobile phases. The elution procedure was as follows: 85 % B-30 % B (0-7 min) and 30 % B-30 % B (7.1-8 min). The flow rate was 0.3 mL/min. The column temperature was 40 ℃, and the injection volume was 10 μL. The main analytical parameters of paeoniflorin, geniposide, saikosaponin b2, liquiritin, paeonol and atractylenolide Ⅲ were m/z 525→449, m/z 433→224, m/z 780→617, m/z 417→254, m/z 167→43 and m/z 249→231, respectively. Ethyl acetate was used to extract the analytes in the plasma. Standard calibration curves of six analytes showed satisfactory linearity (r2≥0.99 2) within the determined ranges. The lower limits of quantification were 0.5 ng/mL for paeoniflorin and liquiritin, 2.5 ng/mL for geniposide and saikosaponin b2 and 1.0 ng/mL for atractylenolide Ⅲ and paeonol, respectively. The intra-day and inter-day precision (RSD %) were all below 6.94 %, and the intra-day and inter-day accuracy (RE %) were within ± 6.10 %. The recovery and ME of six analytes were 85.99 %-98.10 % and 95.78%-108.06%, respectively. Additionally, the method we established in this experiment can be successfully used to study the pharmacokinetics of paeoniflorin and geniposide in beagle plasma.

中文翻译：

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HPLC-MS / MS同时测定比格犬血浆中丹脂逍遥丸的六种成分，单剂给药后pa药苷和子苷的药代动力学研究。

本研究旨在建立可靠，简单的高效液相色谱-串联质谱法（HPLC-MS / MS），以检测比格犬血浆中的eon药苷，子苷，皂苷b2，脂蛋白、,药酚和白术内酯Ⅲ，并研究study药苷和and药的药代动力学。单次服用丹芝逍遥丸（DZXY）后的gen子苷。使用Agilent C18色谱柱进行色谱分离，并使用多反应监测（MRM）模式。使用梯度洗脱程序，溶剂A（乙腈）和溶剂B（0.1％甲酸-水）作为流动相。洗脱步骤如下：85％B-30％B（0-7分钟）和30％B-30％B（7.1-8分钟）。流速为0.3mL / min。柱温为40℃，进样量为10μL。eon药苷的主要分析参数 gen子苷，皂苷b2，脂蛋白，丹皮酚和白术内酯Ⅲ为m / z 525→449，m / z 433→224，m / z 780→617，m / z 417→254，m / z 167→43和m / z分别为249→231。乙酸乙酯用于提取血浆中的分析物。在确定的范围内，六种分析物的标准校准曲线显示出令人满意的线性（r2≥0.992）。eon药苷和脂蛋白的定量下限分别为0.5 ng / mL，子苷和皂苷b2的定量下限为2.5 ng / mL，白术内酯Ⅲ和丹皮酚的定量下限分别为1.0 ng / mL。日内和日间精度（RSD％）均低于6.94％，日间和日间精度（RE％）均在±6.10％以内。六种分析物的回收率和ME分别为85.99％-98.10％和95.78％-108.06％。另外，