Circular RNA (circRNA) is a looped noncoding RNA with a stable structure and tissue-specific expression and widely reported to regulate cancer initiation and progression. However, the circRNA expression patterns and their roles in osteosarcoma initiation and progression are still poorly understood. In this study, we characterized the landscape of circRNAs in osteosarcoma (OS) cell lines, and calculated the epithelial-mesenchymal transition (EMT) scores for OS cell lines. The differential expression analysis revealed that the EMT-related genes were significantly upregulated in the OS cell lines with higher metastatic potentials, and some inflammation-related pathways and pathways involved in cell-cell communications were enriched by these upregulated genes. Furthermore, we constructed a circRNA-based competing endogenous RNA (ceRNA) network, which consisted of 5 circRNAs, 17 miRNAs, and 73 mRNAs. Particularly, hsa_circ_0085360, which had the highest correlation with TRPS1, were characterized by some cancer-related pathways, and TRPS1 and its target gene FGFR3 were closely associated with both event-free survival and overall survival of OS, indicating that hsa_circ_0085360 might have the potential to predict the OS prognosis. In summary, we profiled the circRNA expression patterns in OS, predicted their functionality, and explored the underlying mechanism and prognostic values, which might provide some evidences for OS-related circRNA researches.

中文翻译：

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骨肉瘤细胞系中环状 RNA 的综合表征。

环状 RNA (circRNA) 是一种环状非编码 RNA，具有稳定的结构和组织特异性表达，被广泛报道可调节癌症的发生和进展。然而，circRNA 表达模式及其在骨肉瘤发生和发展中的作用仍然知之甚少。在本研究中，我们描述了骨肉瘤 (OS) 细胞系中 circRNA 的特征，并计算了 OS 细胞系的上皮间质转化 (EMT) 评分。差异表达分析显示，EMT相关基因在具有较高转移潜能的OS细胞系中显着上调，并且这些上调基因丰富了一些炎症相关通路和参与细胞-细胞通讯的通路。此外，我们构建了一个基于 circRNA 的竞争性内源 RNA (ceRNA) 网络，由 5 个 circRNA、17 个 miRNA 和 73 个 mRNA 组成。特别是与TRPS1相关性最高的hsa_circ_0085360具有一些癌症相关通路的特征，TRPS1及其靶基因FGFR3与OS的无事件生存期和总生存期密切相关，表明hsa_circ_0085360可能具有潜力预测 OS 预后。总之，我们分析了 OS 中的 circRNA 表达模式，预测了它们的功能，并探索了潜在的机制和预后价值，这可能为 OS 相关的 circRNA 研究提供一些证据。TRPS1 及其靶基因 FGFR3 与 OS 的无事件生存期和总生存期密切相关，表明 hsa_circ_0085360 可能具有预测 OS 预后的潜力。总之，我们分析了 OS 中的 circRNA 表达模式，预测了它们的功能，并探索了潜在的机制和预后价值，这可能为 OS 相关的 circRNA 研究提供一些证据。TRPS1 及其靶基因 FGFR3 与 OS 的无事件生存期和总生存期密切相关，表明 hsa_circ_0085360 可能具有预测 OS 预后的潜力。总之，我们分析了 OS 中的 circRNA 表达模式，预测了它们的功能，并探索了潜在的机制和预后价值，这可能为 OS 相关的 circRNA 研究提供一些证据。