An innovative strong hydrophilic organic polymer monolithic column of poly(N-benzylquininium chloride-co-1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine) (poly(NBQ-co-TAT)) has been successfully synthesized through in situ copolymerization for capillary electrochromatography. The amphiphilic monomer NBQ and the strong polar cross-linker TAT are firstly used in hydrophilic electrochromatography by taking advantage of the exhibition of hydrophilicity at lower levels of organic solvent and ease formation of porous structure. The monolithic column poly(NBQ-co-TAT) shows powerful hydrophilic selectivity with mobile phase containing more than 60% organic solvent. The introduction of NBQ and TAT enlarges the sources of functional monomers and cross-linkers for HILIC. Due to the presence of the positively charged group in NBQ, an anodic electroosmotic flow is generated with the change of pH values from 2.0 to 12.0. The monolithic column was used for the separations of thioureas, phenols, xanthines, nucleobases, acidic substances and pharmaceuticals. The highest column efficiency for N, N'-dimethylthiourea is 1.15 × 105 N m-1. The application of the monolithic column for a real sample, cytochrome C digestion indicates its great potential in practical application.

中文翻译：

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用两亲性苄基奎宁官能化的强亲水整体柱，用于毛细管电色谱分析和在药物分析中的应用。

聚（N-苄基氯化奎宁-co-1，3，5-三丙烯酰基六氢-1，3，5-三嗪）（聚（NBQ-co-TAT））的创新强亲水有机聚合物整体柱已通过原位共聚用于毛细管电色谱。两亲性单体NBQ和强极性交联剂TAT首先通过在较低有机溶剂含量下表现出亲水性并易于形成多孔结构而首次用于亲水电色谱中。整体柱式聚（NBQ-co-TAT）表现出强大的亲水选择性，流动相中有机溶剂含量超过60％。NBQ和TAT的引入扩大了HILIC的功能单体和交联剂的来源。由于NBQ中存在带正电荷的基团，pH值从2.0变为12.0时，会产生阳极电渗流。整体式色谱柱用于分离硫脲，酚，黄嘌呤，核碱基，酸性物质和药物。N，N'-二甲基硫脲的最高柱效为1.15×105 N m-1。整体柱在实际样品中的应用，细胞色素C的消化表明它在实际应用中具有巨大的潜力。