Abstract Arabinoxylan is an important hemicellulose potentially affecting wheat baking qualities, barley malt quality, and may impart prebiotic benefits. Water-extractable (WEAX) and total arabinoxylan (TOAX) were characterized in 204 wheat and barley accessions, respectively, using an orcinol-ferric chloride assay. WEAX measurement accuracy was optimum when glucose concentration was greater than 12–13 times pentose concentration. The modified method removed enough excess glucose spectral absorbance to make the corrected and uncorrected lines different, but still significantly correlated (p = 0.009, R = 0.94). Mean WEAX (expressed as percentage WEAX of TOAX) for the wheat accessions was 15.90%, ranging from 8.82% to 24.87%, and for barley accessions WEAX the mean was 7.10%, and ranged from 2.98% to 13.86%. Conclusions are 1) the assay is useful for a breeding program because of its semi-high throughput design for the simultaneous analysis of 16 (WEAX) to 40 (TOAX) barley lines or 24 (WEAX) to 40 (TOAX) wheat lines, 2) the trichromatic measurement reduces the impact of glucose, and 3) the broad range of WEAX measured showed that barley and wheat accessions vary in their extractable and unextractable components.

中文翻译：

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Orcinol-氯化​​铁比色法在大麦和小麦种质中水提取物和总阿拉伯木聚糖中的应用

摘要阿拉伯木聚糖是一种重要的半纤维素，可能会影响小麦烘焙品质、大麦麦芽品质，并可能赋予益生元益处。水提取物 (WEAX) 和总阿拉伯木聚糖 (TOAX) 分别在 204 个小麦和大麦种质中使用 orcinol-氯化​​铁测定进行表征。当葡萄糖浓度大于戊糖浓度的 12-13 倍时，WEAX 测量精度最佳。修改后的方法去除了足够多的多余葡萄糖光谱吸光度，使校正线和未校正线不同，但仍显着相关（p = 0.009，R = 0.94）。小麦种质的平均 WEAX（以 TOAX 的 WEAX 百分比表示）为 15.90%，范围为 8.82% 至 24.87%，而大麦种质 WEAX 的平均值为 7.10%，范围为 2.98% 至 13.86%。