Abstract The objective of this study was to apply cuttlefish (Sepia officinalis) skin protein isolate (CSPI) and hydrolysates (CSPH), using commercial Savinase® and Purafect® enzymes, as bioactive additives in the elaboration of gelatin-based films. CSPH and CSPI enriched films were colored and exhibited a higher UV-barrier properties compared to gelatin film. In addition, compared to CSPI added film, an increase of the glass transition temperature by 20 % and 4 %, respectively, for Purafect and Savinase hydrolysates enriched films was noted. However, elongation at break decreased significantly for CSPH incorporated films by 2.5-fold. The tensile strength was reduced by 28.2 % and 44.4 % for Purafect and Savinase hydrolysates added films, respectively. Furthermore, a decrease of water contact angle by 45 % and 51 % for films added with Purafect and Savinase hydrolysates, respectively, was displayed compared to gelatin film. Interestingly, CSPH enriched films also displayed higher antioxidant potential than control gelatin films evaluated by several in vitro assays.

中文翻译：

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基于墨鱼 (Sepia officinalis) 皮肤明胶与墨鱼蛋白水解物的生物活性薄膜：物理化学表征和抗氧化特性

摘要 本研究的目的是将乌贼 (Sepia officinalis) 皮肤分离蛋白 (CSPI) 和水解物 (CSPH)，使用商业 Savinase® 和 Purafect® 酶，作为生物活性添加剂，用于制作基于明胶的薄膜。与明胶薄膜相比，富含 CSPH 和 CSPI 的薄膜被着色并表现出更高的紫外线阻隔性能。此外，与添加 CSPI 的薄膜相比，Purafect 和 Savinase 水解物富集薄膜的玻璃化转变温度分别提高了 20% 和 4%。然而，加入 CSPH 的薄膜的断裂伸长率显着降低了 2.5 倍。添加 Purafect 和 Savinase 水解产物的薄膜的拉伸强度分别降低了 28.2% 和 44.4%。此外，与明胶薄膜相比，添加了 Purafect 和 Savinase 水解物的薄膜的水接触角分别降低了 45% 和 51%。有趣的是，富含 CSPH 的薄膜还显示出比通过几种体外试验评估的对照明胶薄膜更高的抗氧化潜力。