Trichinellosis caused by Trichinella spiralis (T. spiralis) is an important public health problem. DNase II is an acidic endonuclease that catalyzes the degradation of DNA into oligonucleotides. DNase II-7 has been detected at the adult stage of T. spiralis and has been examined in excretory/secretory products. Previous studies have indicated that the DNase II-7 recombinant protein has a high rate of protection against T. spiralis infection in mice. In the present study, the protective effect of DNase II-7 recombinant protein against T. spiralis infection in Large White pigs was further explored. The humoral and cellular immune responses to the DNase II-7 recombinant protein were evaluated, including the dynamic trends of specific IgG, IgG1, IgG2a and IgM antibodies levels, as well as the levels of Th1 (IFN-γ and IL-2) and Th2 (IL-10 and IL-4) cytokines in serum. Our results showed that a Th1 dominated Th1/Th2 mixed immune response was induced by the DNase II-7 recombinant protein for all the time or a short period after vaccination. And the DNase II-7 recombinant protein induced partial protection against T. spiralis infection in pigs, compared to the control group. Our results showed that the DNase II-7 recombinant protein group displayed a 45.7 % reduction in the muscle larvae burden five weeks after being challenged. This study suggested that DNaseII-7 recombinant protein could be used as a potential candidate vaccine against T. spiralis infection in pigs.

由旋毛虫（ T.spiralis ）引起的旋毛虫病是一个重要的公共卫生问题。 DNase II 是一种酸性核酸内切酶，可催化 DNA 降解为寡核苷酸。 DNase II-7 已在旋毛虫成虫阶段检测到，并在排泄/分泌产物中进行了检查。此前的研究表明，DNase II-7重组蛋白对小鼠体内的旋毛虫感染具有很高的保护率。本研究进一步探讨了DNase II-7重组蛋白对大白猪螺旋毛虫感染的保护作用。评估对 DNase II-7 重组蛋白的体液和细胞免疫反应，包括特异性 IgG、IgG1、IgG2a 和 IgM 抗体水平的动态趋势，以及 Th1（IFN-γ 和 IL-2）和血清中的 Th2（IL-10 和 IL-4）细胞因子。我们的结果表明，DNase II-7 重组蛋白在疫苗接种后始终或短时间内诱导了 Th1 主导的 Th1/Th2 混合免疫反应。与对照组相比，DNase II-7 重组蛋白诱导猪抵抗螺旋毛虫感染的部分保护作用。我们的结果表明，DNase II-7 重组蛋白组在受到攻击五周后，肌肉幼虫负荷减少了 45.7%。这项研究表明 DNaseII-7 重组蛋白可作为猪螺旋毛虫感染的潜在候选疫苗。