Protection against Trichinella spiralis in BALB/c mice via oral administration of recombinant Lactobacillus plantarum expressing murine interleukin-4.,Veterinary Parasitology

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Protection against Trichinella spiralis in BALB/c mice via oral administration of recombinant Lactobacillus plantarum expressing murine interleukin-4.
Veterinary Parasitology ( IF 2.0 ) Pub Date : 2020-03-02 , DOI: 10.1016/j.vetpar.2020.109068
Dan Wang ₁ , Qing-Long Gong ₁ , Hai-Bin Huang ₁ , Wen-Tao Yang ₁ , Chun-Wei Shi ₁ , Yan-Long Jiang ₁ , Jian-Zhong Wang ₁ , Yuan-Huan Kang ₁ , Quan Zhao ₁ , Gui-Lian Yang ₁ , Chun-Feng Wang ₁

Affiliation

Interleukin-4 (IL-4), an immunomodulatory cytokine derived from activated T lymphocytes were shown to regulate Th2-type immune responses. It plays an important role in anti-parasitic infections. In this study, a recombinant plasmid was designed using murine IL-4 co-expressed with pgsA anchor system of Lactobacillus plantarum NC8 and was delivered by live Lactobacillus plantarum NC8, which exhibited an enhanced immunogenicity in protection of BALB/c mice from infection with Trichinella spiralis. The results showed that the levels of serum IgG1 and mucosal secretory IgA (sIgA) were both increased significantly in mice orally inoculated with NC8-pSIP409-pgsA-mIL-4, and the Th2 phenotype immune response was up-regulated. A 29.9 % reduction in adult worm burden at 7 days post-infection (dpi) and 83.3 % reduction in muscle larvae burden at 28 dpi were observed in immune-stimulated mice with NC8-pSIP409-pgsA-mIL-4. Moreover, weight loss and pathological changes were also improved in mice of NC8-pSIP409-pgsA-mIL-4 group. Taken together, it suggests that NC8-pSIP409-pgsA-mIL-4 could improve the intestinal mucosal immunity and promoted the elimination of the adult worm in Trichinella-infected mice. This study laid the foundation for the development of a novel vaccines against Trichinellosis.

中文翻译：

通过口服施用表达鼠白细胞介素4的重组植物乳杆菌，对BALB / c小鼠中的旋毛虫进行保护。

白细胞介素4（IL-4），一种由活化T淋巴细胞衍生的免疫调节细胞因子，可调节Th2型免疫应答。它在抗寄生虫感染中起着重要作用。在这项研究中，使用与植物乳杆菌NC8的pgsA锚定系统共表达的鼠IL-4设计重组质粒，并由活的植物乳杆菌NC8递送，它在保护BALB / c小鼠免受旋毛虫感染方面显示出增强的免疫原性。螺旋藻。结果表明，口服NC8-pSIP409-pgsA-mIL-4的小鼠，血清IgG1和粘膜分泌性IgA（sIgA）均显着增加，并且Th2表型免疫应答被上调。感染后7天（dpi）和83天，成虫蠕虫负担减少29.9％。在使用NC8-pSIP409-pgsA-mIL-4免疫刺激的小鼠中，观察到28 dpi的肌肉幼虫负担减少了3％。此外，NC8-pSIP409-pgsA-mIL-4组的小鼠的体重减轻和病理变化也得到改善。两者合计，这表明NC8-pSIP409-pgsA-mIL-4可以改善肠道旋毛虫感染小鼠的肠道粘膜免疫力并促进其消除成虫。这项研究为开发新型抗旋毛虫病疫苗奠定了基础。

更新日期：2020-03-20

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