The present study investigates the efficacy of dimehtlyformamide (DMF) as a permeable cryoprotectant and its effect on quality and fertility of Indian red jungle fowl sperm. Semen was collected from eight mature roosters, pooled, divided into five aliquots and diluted with red fowl extender having DMF (0%, 4%, 6%, 8% and 10%). Diluted semen samples were cooled from 37 °C to 4 °C, 20% glycerol added to control (0% DMF), equilibrated for 10 min and filled in 0.5 mL French straws, kept over liquid nitrogen vapors for 10 min and plunged into liquid nitrogen. Sperm motility, plasma membrane functionality, viability and acrosome integrity were assessed at post dilution, cooling, equilibration and freeze-thawing stage of cryopreservation. Cryopreservation stages had negative effects (P < 0.05) on semen quality parameters. Percentages of sperm motility, plasma membrane functionality, viability and acrosome integrity were recorded highest in extender having 8% DMF at post-dilution, cooling, equilibration and freeze-thawing stage. Fertility results after artificial insemination were recorded higher (P < 0.05) with 8% DMF compared to 20% glycerol. Dimehtlyformamide (8%) in red fowl extender improves the post thaw semen quality and fertility in Indian red jungle fowl and can be used effectively to avoid the contraceptive effects of glycerol.

中文翻译：

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二甲基甲酰胺对印度红原鸡（Gallus gallus murghi）精子质量和受精能力的影响

本研究调查了二甲基甲酰胺 (DMF) 作为渗透性冷冻保护剂的功效及其对印度红原鸡精子质量和生育力的影响。从八只成熟公鸡收集精液，合并，分成五等份，并用含有 DMF（0%、4%、6%、8% 和 10%）的红鸡稀释剂稀释。稀释的精液样品从 37 °C 冷却至 4 °C，向对照中加入 20% 甘油（0% DMF），平衡 10 分钟并装入 0.5 mL 法式吸管，在液氮蒸气上保持 10 分钟并投入液体中氮。在冷冻保存的后稀释、冷却、平衡和冻融阶段评估精子活力、质膜功能、活力和顶体完整性。冷冻保存阶段对精液质量参数有负面影响（P < 0.05）。精子活力的百分比，在后稀释、冷却、平衡和冻融阶段，含有 8% DMF 的增量剂中的质膜功能、活力和顶体完整性记录最高。与 20% 甘油相比，8% DMF 人工授精后的生育率结果更高（P < 0.05）。红鸡稀释剂中的二甲基甲酰胺 (8%) 可改善印度红原鸡解冻后的精液质量和生育能力，可有效避免甘油的避孕作用。