Germplasm preservation of animals, whether they are valuable domestic breeds or rare species, is the main goal of gamete cryobanking. Dogs and cats act as models for this purpose thanks to the wide availability of biological material which can be employed to experiment protocols that can then be applied to wild animals. This review is focused on spermatozoa, oocytes and gonadal tissues cryobanking in small domestic animals, which is still an unsolved case. Like in a courtroom, evidences of cryoinjuries affecting cellular structures will be presented, penalties as loss of functionality due to cellular alterations will be described, and appeal as strategies to protect gametes from damages or rescue their functionality will be discussed. Differences and similarities between single cell or tissue cryopreservation will be highlighted, together with the rationale for the choice of one type of preservation or another and the fundamental principles which they are based on. The deep analysis of different aspects that still hamper the success of cryopreservation in small animals can help clarify where research is most needed. Therefore, as in a cold case, investigation should remain open in order to hopefully find the solution and make these procedures more and more efficient in the future.

中文翻译：

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冷案例：小动物游戏 冷冻银行

动物的种质保存，无论是有价值的家养品种还是稀有物种，都是配子冷冻库的主要目标。狗和猫为此目的充当模型，这要归功于生物材料的广泛可用性，这些材料可用于实验方案，然后可以应用于野生动物。本综述的重点是小型家畜的精子、卵母细胞和性腺组织冷冻库，这仍然是一个悬而未决的案例。就像在法庭上一样，将提供影响细胞结构的冷冻损伤的证据，将描述由于细胞改变导致功能丧失的处罚，并将讨论作为保护配子免受损害或挽救其功能的策略的上诉。将突出显示单细胞或组织冷冻保存之间的差异和相似之处，以及选择一种或另一种保存方式的基本原理以及它们所基于的基本原则。对仍然阻碍小动物冷冻保存成功的不同方面进行深入分析，可以帮助澄清哪些方面最需要研究。因此，就像在一个冷案中一样，调查应该保持开放，以便有希望找到解决方案并使这些程序在未来越来越有效。