This study examined the effect of minocycline alone and in combination with immunotherapy against pythiosis. Twenty rabbits, aged three months old and subcutaneously inoculated with Pythium insidiosum zoospores were divided into four groups (n = 5): treated with minocycline (10 mg/kg/day twice daily), treated with immunotherapy (34 mg subcutaneously every 14 days), treated with minocycline plus immunotherapy, and untreated (control group). The treatments were started 30 days after inoculation and continued for 70 days. The subcutaneous nodular injury areas in infected groups were measured every seven days after the beginning of treatment. Only the rabbits that developed lesions were selected for this study. When compared with the control group over 70 days, the minocycline and minocycline plus immunotherapy groups of rabbits with pythiosis showed significantly reduced injuries. The histopathology showed the presence of inflammation, macrophages and eosinophils. Grocott’s staining revealed irregular hyphae-like structures that were ramified and occasionally septate. Our results suggest that minocycline has fungistatic activity and that the combination of minocycline and immunotherapy is more effective than the individual therapies tested.

这项研究检查了单独使用美满霉素和联合使用抗吡虫啉免疫疗法的效果。年龄为三个月大的兔子20只，皮下接种了腐霉游动孢子分为四组（n = 5）：用米诺环素治疗（10 mg / kg /天，每天两次），用免疫疗法治疗（每14天皮下注射34 mg），用米诺环素加免疫疗法治疗，以及未治疗（对照组） 。接种后30天开始治疗，并持续70天。开始治疗后每隔7天测量感染组的皮下结节损伤区域。仅将发生损伤的兔子用于该研究。与对照组相比，在超过70天的时间里，美托环素和美满环素加免疫治疗组的脓毒症兔子显示出明显减轻的伤害。组织病理学表明存在炎症，巨噬细胞和嗜酸性粒细胞。Grocott的染色显示不规则的菌丝样结构，其分支并偶尔分隔。我们的结果表明，米诺环素具有抑菌活性，并且米诺环素与免疫疗法相结合比单独测试的疗法更有效。