Abstract Soil type and domestication are the main factors that contribute to the determination of root-associated bacterial communities in soybean; however, to what extent these factors influence root-associated fungal communities remains unclear. Here, we grew wild soybean (Glycine soja) and cultivated soybean (Glycine max) in three soil suspensions (black, red and saline-sodic soil) and analyzed the soybean rhizosphere fungal internal transcribed spacer 1 (ITS1) region by high-throughput sequencing technology. The results showed that the fungal communities had significantly different richness and alpha-diversity indexes in the three soil suspensions; however, there was no marked difference in the rhizosphere fungal communities between the two wild and cultivated soybean varieties. Furthermore, soil type was a main factor in determining the distribution of arbuscular mycorrhizal fungi (AMF; Paraglomus and Rhizophagus) and soybean pathogenic fungi (Fusarium and Rhizoctonia). In addition, the abundant rhizosphere fungi from wild soybean exhibited diverse potential functions, while the functions of rhizosphere fungi from cultivated soybean were mainly related to plant nutrient uptake. The diversity and structure of the soybean rhizosphere fungal communities were mainly affected by soil type rather than soybean variety, and domestication caused wild and cultivated soybeans to recruit different rhizosphere fungi. Accordingly, a thorough understanding of soybean fungal communities and their influencing factors is important for improving the health and harvest of soybean.

中文翻译：

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在三种不同土壤悬浮液中生长的野生和栽培大豆的根际真菌群落

摘要 土壤类型和驯化是决定大豆根系相关细菌群落的主要因素；然而，这些因素在多大程度上影响与根相关的真菌群落尚不清楚。在这里，我们在三种土壤悬浮液（黑色、红色和盐碱土）中种植了野生大豆（Glycine soja）和栽培大豆（Glycine max），并通过高通量测序分析了大豆根际真菌内部转录间隔区 1（ITS1）区域技术。结果表明，三种土壤悬浮液中真菌群落的丰富度和α-多样性指数存在显着差异；然而，两个野生和栽培大豆品种之间的根际真菌群落没有显着差异。此外，土壤类型是决定丛枝菌根真菌（AMF；Paraglomus and Rhizophagus）和大豆病原真菌（Fusarium and Rhizoctonia）分布的主要因素。此外，野生大豆中丰富的根际真菌表现出多种潜在功能，而栽培大豆中丰富的根际真菌的功能主要与植物养分吸收有关。大豆根际真菌群落的多样性和结构主要受土壤类型而不是大豆品种的影响，驯化导致野生和栽培大豆招募不同的根际真菌。因此，彻底了解大豆真菌群落及其影响因素对于改善大豆的健康和收获非常重要。Paraglomus 和 Rhizophagus）和大豆病原真菌（Fusarium 和 Rhizoctonia）。此外，野生大豆中丰富的根际真菌表现出多种潜在功能，而栽培大豆中丰富的根际真菌的功能主要与植物养分吸收有关。大豆根际真菌群落的多样性和结构主要受土壤类型而不是大豆品种的影响，驯化导致野生和栽培大豆招募不同的根际真菌。因此，彻底了解大豆真菌群落及其影响因素对于改善大豆的健康和收获非常重要。Paraglomus 和 Rhizophagus）和大豆病原真菌（Fusarium 和 Rhizoctonia）。此外，野生大豆中丰富的根际真菌表现出多种潜在功能，而栽培大豆中丰富的根际真菌的功能主要与植物养分吸收有关。大豆根际真菌群落的多样性和结构主要受土壤类型而不是大豆品种的影响，驯化导致野生和栽培大豆招募不同的根际真菌。因此，彻底了解大豆真菌群落及其影响因素对于改善大豆的健康和收获非常重要。而栽培大豆根际真菌的功能主要与植物养分吸收有关。大豆根际真菌群落的多样性和结构主要受土壤类型而不是大豆品种的影响，驯化导致野生和栽培大豆招募不同的根际真菌。因此，彻底了解大豆真菌群落及其影响因素对于改善大豆的健康和收获非常重要。而栽培大豆根际真菌的功能主要与植物养分吸收有关。大豆根际真菌群落的多样性和结构主要受土壤类型而不是大豆品种的影响，驯化导致野生和栽培大豆招募不同的根际真菌。因此，彻底了解大豆真菌群落及其影响因素对于改善大豆的健康和收获非常重要。