Abstract Microorganisms with a high natural ability to degrade xenobiotics, which are usually characterized by a diverse metabolism and unique features, can be used as natural biocatalysts in wastewater treatment or bioaugmentation processes. The immobilization of such strains greatly increases their stability and degradation properties. The most critical issues in designing whole-cell immobilization systems are selecting the suitable carrier and determining the bacterial features that will promote effective immobilization. In this study, we immobilized the paracetamol-degrading Pseudomonas moorei KB4 strain on the bacterial cellulose disks that were produced by Komagataeibacter xylinus E-89370. The KB4 strain immobilized on the cellulose degraded 150 mg L−1 of paracetamol in three series of 50 mg L−1 in each cycle. The average protein concentration and dehydrogenase activity increased after the degradation of each dose. The specific activity (U mg−1 of protein) of the main enzymes involved in the degradation pathway was 0.22 for deaminase, 5.1 for acylamidohydrolase and 3.49 for ring-cleavage hydroquinone 1,2-dioxygenase. The relative expression level of the genes encoding deaminases and acylamidohydrolases increased in the presence of paracetamol, though more prominently in the immobilized than in the free cells.

中文翻译：

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用于降解扑热息痛的莫雷假单胞菌 KB4 菌株的细菌纤维素全细胞固定系统

摘要 具有高度天然降解异生物质能力的微生物，通常具有多种代谢和独特特征，可用作废水处理或生物强化过程中​​的天然生物催化剂。这些菌株的固定化大大增加了它们的稳定性和降解特性。设计全细胞固定系统的最关键问题是选择合适的载体并确定将促进有效固定的细菌特征。在这项研究中，我们将降解扑热息痛的 Pseudomonas moorei KB4 菌株固定在由 Komagataeibacter xylinus E-89370 产生的细菌纤维素盘上。固定在纤维素上的 KB4 菌株在每个循环中以三个系列的 50 mg L-1 降解了 150 mg L-1 的扑热息痛。每个剂量降解后平均蛋白质浓度和脱氢酶活性增加。参与降解途径的主要酶的比活性（蛋白质的 U mg-1）对于脱氨酶为 0.22，对于酰氨基水解酶为 5.1，对于开环氢醌 1,2-双加氧酶为 3.49。在扑热息痛存在下，编码脱氨酶和酰氨基水解酶的基因的相对表达水平增加，但在固定细胞中比在游离细胞中更显着。