Although bone defects can be restored spontaneously, bone reconstruction with sufficient strength and volume continues to be a challenge in clinical practices. In recent years, the use of a variety of biomaterials with bioactivity has been attempted to compensate for this limitation. Herein, we fabricated a pDNA (encoding for BMP-2)-loaded asymmetrically porous polycaprolactone (PCL)/Pluronic F127 membrane as a bioactive guided bone regeneration (GBR) membrane, using a modified immersion-precipitation method. It was observed that the GBR membrane allows continuous release of pDNA for more than 20 weeks. The pDNA was sufficiently transfected into human bone marrow stem cells (hBMSCs) without significant cytotoxicity and the gene-transfected cells showed prolonged synthesis of BMP-2. From in vitro osteogenic differentiation and in vivo animal studies, the effective induction of osteogenic differentiation of hBMSCs and enhanced bone regeneration by the pDNA-loaded asymmetrically porous PCL/Pluronic F127 membrane was observed, suggesting that the pDNA-loaded membrane as a bioactive GBR membrane can be an alternative therapeutic technique for effective bone regeneration.

尽管可以自发修复骨缺损，但是具有足够强度和体积的骨重建仍然是临床实践中的挑战。近年来，已经尝试使用具有生物活性的多种生物材料来弥补这一限制。在这里，我们使用改进的浸没-沉淀法制备了pDNA（编码BMP-2）负载的不对称多孔聚己内酯（PCL）/ Pluronic F127膜作为生物活性骨再生（GBR）膜。观察到，GBR膜可使pDNA连续释放超过20周。将pDNA充分转染到人骨髓干细胞（h BMSCs）中，而没有明显的细胞毒性，并且基因转染的细胞显示出BMP-2的合成时间延长。从体外成骨细胞分化和体内动物研究，观察到pDNA负载不对称多孔PCL / Pluronic F127膜可有效诱导h BMSCs的成骨分化并增强骨再生，这表明pDNA负载膜可作为具有生物活性的GBR膜。有效骨再生的另一种治疗技术。