Abstract Tanshinones are compounds of diterpenoid, which are used in the treatment of cardiovascular diseases; however there is an urgent need to improve their production using biotechnological methods. In the present study, Agrobacterium-mediated transformation was used to produce hairy roots of Salvia miltiorrhiza expressing kaurene synthase-like (SmKSL), a tanshinone biosynthesis gene. The results revealed that the yield of total tanshinone increased by a maximum of 2.7-fold in the transgenic Salvia miltiorrhiza hairy roots overexpressing SmKSL as compared with wild-type control lines. Treatment with elicitors further increased the total tanshinone production in the SmKSL-overexpressing lines. Yeast extract treatment of line p35S::SmKSL-15 produced the highest total tanshinone content (4.2 mg/g dry weight), representing an increase of 3.5-fold compared with that in the wild-type control lines. At the same time, qRT-PCR analysis indicated that tanshinone accumulation correlated positively with the transcriptional level of the two pathway genes.

中文翻译：

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SmKSL过表达结合诱导剂处理增强丹参毛状根丹参酮的产生

摘要 丹参酮是二萜类化合物，用于治疗心血管疾病；然而，迫切需要使用生物技术方法改进它们的生产。在本研究中，农杆菌介导的转化用于生产丹参的毛状根，表达丹参酮生物合成基因 kaurene synthase-like (SmKSL)。结果表明，与野生型对照品系相比，在过表达 SmKSL 的转基因丹参毛状根中，总丹参酮的产量最多增加了 2.7 倍。用激发子处理进一步增加了 SmKSL 过表达品系中丹参酮的总产量。p35S::SmKSL-15 系的酵母提取物处理产生最高的总丹参酮含量（4.2 毫克/克干重），增加了 3。与野生型对照品系相比增加了 5 倍。同时，qRT-PCR 分析表明丹参酮积累与两个途径基因的转录水平呈正相关。