There is a renewed research interest in food industry in natural additives to improve food shelf life and provide preventive or therapeutic effects on chronic ailments. The aim of this study was to optimise the concentration of three bioactives, rosmarinic, oleanolic and ursolic acids from Lavandula luisieri ethanolic extracts using the supercritical antisolvent fractionation technique. In order to evaluate the influence of pressure and CO₂ flow rate in the process, response surface methodology was employed. Actives quantification was accomplished using HPLC-Photodiode array. Rosmarinic acid, was completely retained and concentrated in the precipitation vessel, while oleanolic and ursolic acids distributed between both fractions. The supercritical antisolvent fractionation process is a useful and green technology to concentrate bioactive in a fine solid to be applied as natural preservative in food products. The optimum conditions for higher mass recovery and concentration of actives were 130 bar and CO₂ feeding flow rate 30 g/min, respectively.

食品工业对天然添加剂的新研究兴趣是提高食品的保质期并提供对慢性疾病的预防或治疗作用。这项研究的目的是使用超临界反溶剂分馏技术优化Lavandula luisieri乙醇提取物中三种生物活性成分（迷迭香酸，齐墩果酸和熊果酸）的浓度。为了评估压力和CO ₂的影响流程中的流速，采用响应面方法。使用HPLC-光电二极管阵列完成活性物质定量。迷迭香酸被完全保留并浓缩在沉淀容器中，而齐墩果酸和熊果酸则分布在两个馏分之间。超临界抗溶剂分馏工艺是一种有用的绿色技术，可将生物活性物浓缩在细小的固体中，用作食品中的天然防腐剂。更高质量回收率和活性物质浓度的最佳条件分别为130 bar和30 g / min的CO ₂进料流速。