Abstract Naringenin, a natural plant flavonoid found in citrus fruits, has been reported to exhibit a wide range of pharmacological functions, including anticancer, antioxidant, antiatherogenic, antithrombotic, and vasodilator activities. Naringenin can be produced from the naringinase (NGase)-catalyzed enzymatic hydrolysis of naringin. However, the poor solubility of naringin in aqueous systems considerably limits the efficiency of naringenin biocatalysis. In this work, a novel substrate adsorption system was proposed for naringin adsorption to increase the efficiency of naringin hydrolysis and naringenin production. Three Amberlite macroporous resins, namely, XAD-4, XAD-7HP and XAD-16, were investigated for their naringin adsorption capacities and effects on NGase hydrolysis. Results indicated that the physical properties of the resins played a critical role in naringin adsorption and naringenin enzymatic synthesis. Naringin hydrolysis was carried out using free and adsorbed substrates. The substrate adsorption strategy could increase the catalytic efficiency at a high naringin concentration. In addition, the reaction conditions for enzymatic naringenin synthesis were optimized, and naringenin was prepared at a liter scale with a high substrate concentration. These results suggested that substrate adsorption is a promising strategy to increase the enzymatic hydrolysis efficiency of naringenin in aqueous systems.

中文翻译：

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柚皮苷酶催化大孔树脂吸附柚皮苷的水解

摘要 柚皮素是柑橘类水果中的一种天然植物黄酮类化合物，据报道具有广泛的药理作用，包括抗癌、抗氧化、抗动脉粥样硬化、抗血栓形成和血管扩张活性。柚皮素可以由柚皮苷酶 (NGase) 催化的柚皮苷酶水解产生。然而，柚皮苷在水性体系中的溶解性较差，极大地限制了柚皮苷生物催化的效率。在这项工作中，提出了一种用于柚皮苷吸附的新型底物吸附系统，以提高柚皮苷水解和柚皮苷生产的效率。研究了三种 Amberlite 大孔树脂，即 XAD-4、XAD-7HP 和 XAD-16，研究了它们的柚皮苷吸附能力和对 NGase 水解的影响。结果表明，树脂的物理性质在柚皮苷吸附和柚皮苷酶促合成中起关键作用。使用游离和吸附底物进行柚皮苷水解。底物吸附策略可以在高柚皮苷浓度下提高催化效率。此外，优化了酶法合成柚皮素的反应条件，以高底物浓度制备了升规模的柚皮素。这些结果表明，底物吸附是提高柚皮素在水性体系中酶水解效率的一种有前景的策略。底物吸附策略可以在高柚皮苷浓度下提高催化效率。此外，优化了酶法合成柚皮素的反应条件，以高底物浓度制备了升规模的柚皮素。这些结果表明，底物吸附是提高柚皮素在水性体系中酶水解效率的一种有前景的策略。底物吸附策略可以在高柚皮苷浓度下提高催化效率。此外，优化了酶法合成柚皮素的反应条件，以高底物浓度制备了升规模的柚皮素。这些结果表明，底物吸附是提高柚皮素在水性体系中酶水解效率的一种有前景的策略。