Abstract It is inherently difficult for a conventional microscopic imaging system to produce images with both wide field of view (FOV) and high-resolution because of its limited space bandwidth product. Fourier ptychographic microscopy (FPM) has been developed to overcome this limitation by using angle-varied illumination to enhance the performance of the system. In this paper, we propose a FPM imaging approach that employs a laser source with a newly developed illumination control optical system. The proposed system consists of a digital micromirror device and a zoom system with a condenser lens to provide sample illumination with angle-varied pattern directions and controllable pattern sizes. In addition, a total internal reflection prism is employed in the system to remove unexpected background noise and extend the FOV for reconstruction. The proposed system is validated against a USAF resolution target and a biological sample, and as a result reconstructs images with higher resolution and more details once the positional misalignment correction algorithm is employed. We envision that the proposed FPM system will be suitable for high-speed imaging applications.

中文翻译：

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基于背景降噪系统的激光傅里叶 ptychographic 显微镜的可调谐照明

摘要 传统的显微成像系统由于其空间带宽积有限，难以产生具有宽视场（FOV）和高分辨率的图像。傅立叶 ptychographic 显微镜 (FPM) 已被开发以通过使用角度变化的照明来提高系统的性能来克服这一限制。在本文中，我们提出了一种 FPM 成像方法，该方法采用具有新开发的照明控制光学系统的激光源。所提出的系统由一个数字微镜装置和一个带有聚光透镜的变焦系统组成，以提供具有角度变化图案方向和可控图案尺寸的样品照明。此外，系统中采用了全内反射棱镜来去除意外的背景噪声并扩展 FOV 以进行重建。所提出的系统针对美国空军分辨率目标和生物样本进行了验证，因此一旦采用位置错位校正算法，就可以以更高分辨率和更多细节重建图像。我们设想所提出的 FPM 系统将适用于高速成像应用。