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Phytochemical analysis of the methanolic leaves extract of Niedenzuella multiglandulosa (Malpighiaceae), a plant species toxic to cattle in Brazil
Phytochemistry Letters ( IF 1.3 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.phytol.2020.02.005
Helena Mannochio Russo , Emerson Ferreira Queiroz , Laurence Marcourt , Adriano Rutz , Pierre-Marie Allard , Rafael Felipe de Almeida , Nilton Marques Carvalho , Jean-Luc Wolfender , Vanderlan da Silva Bolzani

Niedenzuella multiglandulosa (Malpighiaceae) is a neotropical liana responsible for cattle intoxication outbreaks in Brazil that may lead to abortion and even death of the animals, causing substantial losses in Brazilian trade balance. A phytochemical study of N. multiglandulosa leaves was performed in order to obtain chemical information about secondary metabolites of this poorly studied species. The methanolic extract was purified by medium pressure liquid chromatography (MPLC) combined with semi preparative HPLC-PDA leading to the isolation of 16 compounds, including a new steroid named 5-hydroxypodecdysone B (13). The structure of the isolated compounds was determined by classical spectroscopic methods such as NMR and HRMS. Complementary 19F NMR and UPLC-HRMS analysis did not detect the presence of monofluoracetate (MFA), a toxic compound previously described from this species.



中文翻译:

Niedenzuella multiglandulosa(Malpighiaceae)的甲醇叶片提取物的植物化学分析,该植物对巴西的牛有毒

Niedenzuella multiglandulosa(Malpighiaceae)是一种新热带藤本植物,负责巴西的牛中毒暴发,这可能导致动物流产甚至死亡,从而造成巴西贸易平衡的重大损失。为了获得关于此研究欠佳物种的次生代谢产物的化学信息,对多头猪笼草的叶子进行了植物化学研究。通过中压液相色谱(MPLC)与半制备型HPLC-PDA结合纯化甲醇提取液,从而分离出16种化合物,其中包括一种名为5-hydroxypodecdysone B的新类固醇(13)。分离的化合物的结构通过经典的光谱学方法如NMR和HRMS确定。补充19F NMR和UPLC-HRMS分析未检测到一氟乙酸盐(MFA)的存在,一氟乙酸盐是先前描述于该物种的有毒化合物。

更新日期:2020-03-20
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