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Detection of adeABC efllux pump encoding genes and antimicrobial effect of Mentha longifolia and Menthol on MICs of imipenem and ciprofloxacin in clinical isolates of Acinetobacter baumannii.
BMC Complementary and Alternative Medicine Pub Date : 2020-03-19 , DOI: 10.1186/s12906-020-02887-7
Hassan Mahmoudi 1 , Leili Shokoohizadeh 1 , Nayreh Zare Fahim 1 , Ali Mohamadi Bardebari 1 , Shirin Moradkhani 2 , Mohammad Yousef Alikhani 1, 3
Affiliation  

Acinetobacter baumannii is an opportunistic pathogen that causes nosocomial infections especially in patients in intensive care units (ICUs). Accordingly, the aim of our study was to detection of adeABC efllux pump encoding genes and antimicrobial effect of the essential oil of Mentha longifolia and Menthol on the minimum inhibitory concentration (MIC) of imipenem and ciprofloxacin in clinical isolates of A. baumannii. A total of 75 clinical isolates of A. baumannii were collected. The presence of efflux pump genes was detected by polymerase chain reaction (PCR). The minimum inhibitory concentration (MIC) of the essential oil of Mentha longifolia and Menthol and their combined effect with antibiotics were measured by microbroth dilution method and fractional inhibitory concentration (FIC) index. The frequency of adeA, adeB, and adeC genes in clinical isolates of A. baumannii were 86.7, 90.7, and 92%, respectively. When the essential oil of Mentha longifolia was combined with ciprofloxacin and imipenem, MICs decreased 4- and 8-fold, respectively. In the combination of menthol with imipenem, the resistance to imipenem was reduced from 0- to 16-fold in 90% (63/70) of the isolates. The presence of efflux pump genes in more than 90% of A. baumannii isolates indicates its potential role in inducing imipenem- and ciprofloxacin-resistance in this bacterium. Menthol has an antimicrobial effect as an active ingredient in Mentha longifolia. In the future, the combination of medicinal plants with antibiotics can be used as a complement in treating diseases caused by drug-resistant bacteria such as A. baumannii infections.

中文翻译:

鲍曼不动杆菌临床分离株中adeABC外排泵编码基因的检测以及薄荷叶和薄荷醇对亚胺培南和环丙沙星MIC的抑制作用。

鲍曼不动杆菌是一种机会病原体,尤其在重症监护病房(ICUs)的患者中会引起医院感染。因此,我们的研究目的是检测adeABC外排泵编码基因以及薄荷叶和薄荷醇的精油对鲍曼不动杆菌临床分离物中亚胺培南和环丙沙星的最低抑菌浓度(MIC)的影响。总共收集了75种鲍曼不动杆菌临床分离株。通过聚合酶链反应(PCR)检测外排泵基因的存在。通过微泡稀释法和分数抑制浓度(FIC)指数,测定了薄荷脑和薄荷醇精油的最小抑制浓度(MIC)及其与抗生素的联合作用。adeA,adeB,鲍曼不动杆菌临床分离株中的Ade和adeC基因分别为86.7、90.7和92%。当薄荷薄荷精油与环丙沙星和亚胺培南组合使用时,MIC分别降低4倍和8倍。在薄荷醇和亚胺培南的组合中,对亚胺培南的抗性在90%(63/70)的分离物中从0降低到16倍。超过90%的鲍曼不动杆菌分离株中存在外排泵基因,表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。分别。当薄荷薄荷精油与环丙沙星和亚胺培南组合使用时,MIC分别降低4倍和8倍。在薄荷醇和亚胺培南的组合中,对亚胺培南的抗性在90%(63/70)的分离物中从0降低到16倍。超过90%的鲍曼不动杆菌分离株中存在外排泵基因,表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。分别。当薄荷薄荷精油与环丙沙星和亚胺培南组合使用时,MIC分别降低4倍和8倍。在薄荷醇和亚胺培南的组合中,对亚胺培南的抗性在90%(63/70)的分离物中从0降低到16倍。超过90%的鲍曼不动杆菌分离株中存在外排泵基因,表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。分别。在薄荷醇和亚胺培南的组合中,对亚胺培南的抗性在90%(63/70)的分离物中从0降低到16倍。超过90%的鲍曼不动杆菌分离株中存在外排泵基因,表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。分别。在薄荷醇与亚胺培南的组合中,对亚胺培南的抗性在90%(63/70)的分离物中从0降低到16倍。超过90%的鲍曼不动杆菌分离株中存在外排泵基因,表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。鲍曼氏菌分离物表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病的补充,例如鲍曼不动杆菌感染。鲍曼氏菌分离物表明其在该细菌中诱导亚胺培南和环丙沙星耐药的潜在作用。薄荷醇作为薄荷叶中的活性成分具有抗菌作用。将来,药用植物与抗生素的组合可以用作治疗由耐药细菌引起的疾病(例如鲍曼不动杆菌感染)的补充。
更新日期:2020-04-22
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